High-throughput dielectrophoretic cell sorting assisted by cell sliding on scalable electrode tracks made of conducting-PDMS

被引:23
作者
Nie, Xiaofeng [1 ]
Luo, Yuan [2 ,3 ]
Shen, Penghui [1 ]
Han, Chengwu [4 ]
Yu, Duli [1 ,5 ]
Xing, Xiaoxing [1 ]
机构
[1] Beijing Univ Chem Technol, Coll Informat Sci & Technol, 15 North 3rdRing Rd, Beijing 100029, Peoples R China
[2] CALTECH, Dept Med Engn, Pasadena, CA 91125 USA
[3] Southern Univ Sci & Technol, Sch Engn, Dept Biomed Engn, 1088 Xueyuan Ave, Shenzhen 518055, Peoples R China
[4] China Japan Friendship Hosp, Dept Clin Lab, 2 Yinghuayuan East St, Beijing 100029, Peoples R China
[5] Beijing Adv Innovat Ctr Soft Matter Sci & Engn, 15 North 3rdRing Rd, Beijing 100029, Peoples R China
基金
中国国家自然科学基金;
关键词
Dielectrophoresis; Cell separation; High throughput; Conducting-PDMS; Straight track; PARTICLE SEPARATION; MICROFLUIDIC DEVICE; SINGLE-PARTICLE; 3D ELECTRODES; CANCER-CELLS; CHIP;
D O I
10.1016/j.snb.2020.128873
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Dielectrophoresis (DEP) as a label-free cell separation approach in microdevices has been extensively investigated for a variety of applications. 3D microelectrodes made of conducting-PDMS inherit the merit of volumetric electrodes for generating influential DEP force throughout the entire channel depth and meanwhile, exploit low-cost fabrication process by soft lithography. However, the configuration of conducting-PDMS electrodes is limited to being embedded in sidewall of flow chamber, which leads to rather low flow rate and difficulties in extension of the flow rate. We previously reported a more effective configuration with 3D interdigitated electrodes made of silicon that assist cell sliding along solid tracks, yet such device requires expensive silicon dry etching and, moreover, the track appears to be patterned with non-straight and wavy outline, which not only hinders the flow rate but also allows cell sliding to occur only along its downstream side. Here we demonstrate low-cost silver-PDMS electrode-track featuring ideally straight outline that induces rather uniform drag to drive smooth cell sliding. Such design achieves live and dead cell separation at flow rate twice as that of silicon tracks with cell loading concentration 10 times higher. It also fully utilizes the track to enable cell sliding on both of the up- and down-stream sides. Notably, we also demonstrate that this track is expandable to be V-shape for more advanced bidirectional cell sliding, which is showcased here by tumor cells separation from lymphocytes at 1.2 mL/h. Such results greatly enhance the throughput as compared to the state-of-art conducting-PDMS based cell separator.
引用
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页数:11
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