Activation of P388D1 macrophage cell line by chemotherapeutic drugs

被引：10

作者：

Pai, K ^{[1
]}

Shrivastava, A ^{[1
]}

Kumar, R ^{[1
]}

Khetarpal, S ^{[1
]}

Sarmah, B ^{[1
]}

Gupta, P ^{[1
]}

Sodhi, A ^{[1
]}

机构：

[1] BANARAS HINDU UNIV,SCH BIOTECHNOL,VARANASI 221005,UTTAR PRADESH,INDIA

来源：

LIFE SCIENCES | 1997年 / 60卷 / 15期

关键词：

macrophage activation; chemotherapeutic drugs; IL-1; TNF; nitric oxide; lysozyme;

D O I：

10.1016/S0024-3205(97)00068-4

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

It has been found that certain antineoplastic drugs impart their function with a distinct duality. Besides being tumoricidal, they are capable of acting as immunopotentiator. This led us to investigate the effect of cytosine arabinoside (CA), vincristine sulphate (VS), cyclophosphamide (CS), mitomycin C (MMC), hydroxy urea (HU) and lipopolysaccharide (LPS) on a macrophage cell line P388D1. Supernatants collected from P388D1 cells treated with CA, VS, CS, MMC, HU or LPS demonstrated enhanced production of tumor necrosis factor (TNF) confirmed by bioassay on L929 tumor target cells and increased interleukin-l (IL-l) production by standard thymocyte proliferation bioassay. Also, supernatants showed increased amounts of nitric oxide and lysozyme using Griess reaction and reduction in turbidity of Micrococcus lysodeikticus, respectively. The above findings demonstrate that these drugs may be used not only as chemotherapeutic agents but also as macrophage-activating agents.

引用

页码：1239 / 1248

页数：10

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