Time-resolved long-lived infrared emission from bacteriorhodopsin during its photocycle

被引：6
作者：
Wang, JP [1 ]
El-Sayed, MA [1 ]
机构：
[1] Georgia Inst Technol, Sch Chem & Biochem, Laser Dynam Lab, Atlanta, GA 30332 USA
来源：
BIOPHYSICAL JOURNAL | 2002年 / 83卷 / 03期
关键词：
D O I：
10.1016/S0006-3495(02)73928-3
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
The infrared emission observed below 2000 cm(-1) upon exciting retinal in bacteriorhodopsin (bR) is found to have a rise time in the submicrosecond time regime and to relax with two exponential components on the submillisecond to millisecond time scale. These time scales, together with the assignment of this emission to hot vibrations from the all-trans retinal (in bR) and the 13-cis retinal (in the K intermediate), support the recent assignment of the J-intermediate as an electronically excited species (Atkinson et al., J. Phys. Chem. A. 104:4130-4139, 2000) rather than a vibrationally hot K intermediate. A discussion of these time scales of the observed infrared emission is given in terms of the competition between radiative and nonradiative relaxation processes of the vibrational states involved.
引用
收藏
页码：1589 / 1594
页数：6
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