Multicapillary SDS-gel electrophoresis for the analysis of fluorescently labeled mAb preparations: A high throughput quality control process for the production of QuantiPlasma and PlasmaScan mAb libraries

被引：9

作者：

Szekely, Andrea ^{[1
]}

Szekrenyes, Akos ^{[2
]}

Kerekgyarto, Marta ^{[2
]}

Balogh, Attila ^{[1
]}

Kadas, Janos ^{[1
,3
]}

Lazar, Jozsef ^{[1
]}

Guttman, Andras ^{[2
,4
]}

Kurucz, Istvan ^{[1
]}

Takacs, Laszlo ^{[1
,3
]}

机构：

[1] BioSyst Int Kft, H-4032 Debrecen, Hungary

[2] Univ Debrecen, Horvath Lab Bioseparat Sci, Res Ctr Mol Med, Med & Hlth Sci Ctr, Debrecen, Hungary

[3] Univ Debrecen, Dept Human Genet, Med & Hlth Sci Ctr, Monoclonal Antibody Prote Lab, Debrecen, Hungary

[4] Univ Pannonia, MUKKI, MTA PE Translat Glyc Grp, Veszprem, Hungary

来源：

ELECTROPHORESIS | 2014年 / 35卷 / 15期

关键词：

Capillary electrophoresis; Glycoform; mAb proteomics; Quality control; SDS; SODIUM DODECYL-SULFATE; CAPILLARY-ELECTROPHORESIS; MONOCLONAL-ANTIBODIES; MASS-SPECTROMETRY; HETEROGENEITY; GLYCOSYLATION; PROTEINS; STABILITY; CHROMATOGRAPHY; OPTIMIZATION;

D O I：

10.1002/elps.201400208

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Molecular heterogeneity of mAb preparations is the result of various co- and post-translational modifications and to contaminants related to the production process. Changes in molecular composition results in alterations of functional performance, therefore quality control and validation of therapeutic or diagnostic protein products is essential. A special case is the consistent production of mAb libraries (QuantiPlasma (TM) and PlasmaScan (TM)) for proteome profiling, quality control of which represents a challenge because of high number of mAbs (>1000). Here, we devise a generally applicable multicapillary SDS-gel electrophoresis process for the analysis of fluorescently labeled mAb preparations for the high throughput quality control of mAbs of the QuantiPlasma (TM) and PlasmaScan (TM) libraries.

引用

页码：2155 / 2162

页数：8

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