Engineering the Protein Corona Structure on Gold Nanoclusters Enables Red-Shifted Emissions in the Second Near-infrared Window for Gastrointestinal Imaging

被引：107

作者：

Wang, Weili ^{[1
]}

Kong, Yifei ^{[2
]}

Jiang, Jun ^{[1
]}

Xie, Qianqian ^{[1
]}

Huang, Yang ^{[3
]}

Li, Guanna ^{[4
,5
]}

Wu, Di ^{[1
]}

Zheng, Huizhen ^{[1
]}

Gao, Meng ^{[1
]}

Xu, Shujuan ^{[1
]}

Pan, Yanxia ^{[1
]}

Li, Wei ^{[1
]}

Ma, Ronglin ^{[1
]}

Wu, Mei X. ^{[2
]}

Li, Xuehua ^{[3
]}

Zuilhof, Han ^{[4
,5
]}

Cai, Xiaoming ^{[6
]}

Li, Ruibin ^{[1
]}

机构：

[1] Soochow Univ, Collaborat Innovat Ctr Radiol Med Jiangsu Higher, Sch Radiol & Interdisciplinary Sci RAD X, State Key Lab Radiat Med & Protect, Suzhou 215123, Jiangsu, Peoples R China

[2] Harvard Med Sch, Massachusetts Gen Hosp, Wellman Ctr Photomed, Boston, MA 02114 USA

[3] Dalian Univ Technol, Sch Environm Sci & Technol, Key Lab Ind Ecol & Environm Engn, Dalian 116024, Peoples R China

[4] Wageningen Univ, Lab Organ Chem, Stippeneng 4, NL-6703 WE Wageningen, Netherlands

[5] King Abdulaziz Univ, Fac Engn, Dept Chem & Mat Engn, Jeddah, Saudi Arabia

[6] Soochow Univ, Sch Publ Hlth, Suzhou 215123, Jiangsu, Peoples R China

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2020年 / 59卷 / 50期

基金：

中国国家自然科学基金;

关键词：

fluorescent probes; gold nanoparticles; protein corona; red shift; tumor imaging; NANOPARTICLES;

D O I：

10.1002/anie.202010089

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The application of NIR-II emitters for gastrointestinal (GI) tract imaging remains challenging due to fluorescence quenching in the digestive microenvironment. Herein, we report that red-shifting of the fluorescence emission of Au nanoclusters (AuNCs) into NIR-II region with improved quantum yields (QY) could be achieved by engineering a protein corona structure consisting of a ribonuclease-A (RNase-A) on the particle surfaces. RNase-A-encapsulated AuNCs (RNase-A@AuNCs) displayed emissions at 1050 nm with a 1.9 % QY. Compared to rare earth and silver-based NIR-II emitters, RNase-A@AuNCs had excellent biocompatibility, showing >50-fold higher sensitivity in GI tract, and migrated homogenously during gastrointestinal peristalsis to allow visualization of the detailed structures of the GI tract. RNase-A@AuNCs could successfully examine intestinal tumor mice from healthy mice, indicating a potential utility for early diagnosis of intestinal tumors.

引用

页码：22431 / 22435

页数：5

共 27 条

[1]

Antaris AL, 2016, NAT MATER, V15, P235, DOI [10.1038/NMAT4476, 10.1038/nmat4476]

[2]

Bergin Ingrid L, 2013, Int J Biomed Nanosci Nanotechnol, V3, DOI 10.1504/IJBNN.2013.054515

[3] Molecular Mechanisms, Characterization Methods, and Utilities of Nanoparticle Biotransformation in Nanosafety Assessments [J].