Production and characterization of the recombinant Islet Neogenesis Associated Protein (rINGAP)

被引：5
|
作者：
Assouline-Thomas, Beatrice [1 ,2 ]
Pilotte, Amelie [3 ,4 ]
Petropavlovskaia, Maria [1 ,2 ]
Makhlin, Julia [1 ,2 ]
Ding, Jieping [1 ,2 ]
McLeod, David [5 ]
Hanley, Stephen [1 ,2 ]
Massie, Bernard [3 ,4 ,6 ]
Rosenberg, Lawrence [1 ,2 ,7 ]
机构：
[1] McGill Univ, Dept Surg, Montreal, PQ H3G 1A4, Canada
[2] McGill Univ, Ctr Hlth, Ctr Pancreat Dis, Montreal, PQ H3G 1A4, Canada
[3] Natl Res Council Canada, Biotechnol Res Inst, Grp Vecteurs Genom & Therapie Gen, Montreal, PQ H4P 2R2, Canada
[4] Univ Montreal, Dept Microbiol & Immunol, Fac Med, Montreal, PQ H3C 3J7, Canada
[5] Queens Univ, Queens Prot Funct Discovery Facil, Kingston, ON K7L 3N6, Canada
[6] Univ Quebec, INRS IAF, Laval, PQ H7N 4Z3, Canada
[7] Sir Mortimer B Davis Jewish Hosp, Montreal, PQ H3T 1E2, Canada
来源：
PROTEIN EXPRESSION AND PURIFICATION | 2010年 / 69卷 / 01期
关键词：
INGAP; Lentiviral transduction; Islet neogenesis; Recombinant protein; SYRIAN GOLDEN-HAMSTER; BETA-CELL DEFICIT; LENTIVIRAL VECTORS; EXPRESSION; GENE; PENTADECAPEPTIDE; NESIDIOBLASTOSIS; REGENERATION; INDUCTION; APOPTOSIS;
D O I：
10.1016/j.pep.2009.07.009
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Islet Neogenesis Associated Protein (INGAP) is implicated in pancreatic islet neogenesis. INGAP peptide, a pentadecapeptide comprising amino acids 104-118, reverses diabetes in rodents and improves glucose homeostasis in patients with diabetes. The mechanism of INGAP action is unknown, but such studies would benefit from the availability of the full-length recombinant protein (rINGAP). Here we report the production of rINGAP from 293-SF cells following lentiviral transduction, and its characterization by MALDI-TOF and Q-TOF Mass Spectrometry, and HPLC. Importantly, we show that rINGAP exhibits 100x the bioactivity of INGAP peptide on a molar basis in an in vitro assay of human islet regeneration. (C) 2009 Elsevier Inc. All rights reserved.
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页码：1 / 8
页数：8
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