Benchtop and Handheld Energy-Dispersive X-Ray Fluorescence (EDXRF) as Alternative for Selenium Concentration Measurement in Biofortified Broccoli Seedling

被引：7

作者：

Bachiega, Patricia ^{[1
]}

de Almeida, Eduardo ^{[2
]}

Salgado, Jocelem Mastrodi ^{[1
]}

Zezzi Arruda, Marco Aurelio ^{[3
,4
]}

Lehmann, Eraldo Luiz ^{[3
,4
]}

Morzelle, Maressa Caldeira ^{[5
]}

Pereira de Carvalho, Hudson Wallace ^{[2
]}

机构：

[1] Univ Sao Paulo, Esalq, Escola Super Agr Luiz de Queiroz, Dept Agroind Alimentos & Nutr, Ave Padua Dias 111, BR-13418900 Piracicaba, SP, Brazil

[2] Univ Sao Paulo, CENA, LIN, BR-13416000 Piracicaba, Brazil

[3] Univ Estadual Campinas, UNICAMP, GEPAM, Dept Quim Analit,Inst Quim, POB 6154, BR-13083970 Campinas, SP, Brazil

[4] Univ Estadual Campinas, UNICAMP, Inst Quim, Inst Nacl Ciencia & Tecnol Bioanalit, BR-13083970 Campinas, SP, Brazil

[5] Univ Fed Mato Grosso, FANUT, UFMT, Dept Alimentos Nutr,Fac Nutr, Rua Fernando Correa Costa 2367, BR-78060900 Cuiaba, MT, Brazil

来源：

FOOD ANALYTICAL METHODS | 2019年 / 12卷 / 07期

基金：

巴西圣保罗研究基金会;

关键词：

Brassica oleracea; X-ray fluorescence; Complex matrices; Biofortification; Leaves; Selenium; SUPPLEMENTATION; SPECTROMETRY; PERFORMANCE; ENVIRONMENT; METABOLISM; RICE; PXRF;

D O I：

10.1007/s12161-019-01489-5

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Biofortification of crops with selenium (Se) is an effective strategy to increase the consumption of this micronutrient. Inductively coupled plasma mass spectrometry (ICP-MS) is one of the most used techniques in quantifying Se. However, due to some disadvantages (high cost, long analysis time, and being destructive), there is a need for new alternatives to Se quantification. This study aimed at establishing instrumental parameters for Se quantification, using two energy-dispersive X-ray fluorescence spectrometry (EDXRF) techniques (benchtop and handheld) in Se-biofortified broccoli seedlings and comparing it with ICP-MS. The results showed that the selection of proper filters for both EDXRF systems was crucial for determining Se and, when compared with ICP-MS, presented similar results for selenium-biofortified broccoli seedling treatments (BS50Se). In addition, the EDXRF techniques presented a Se limit of detection (LOD) at 0.6-0.9mgkg(-1). This study demonstrates that EDXRF systems were successfully applied to concentration measurement of Se in biofortified samples and linked to low cost and shorter analysis time.

引用

页码：1520 / 1527

页数：8

共 41 条

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