EST-SSR markers from Fragaria vesca L. cv. yellow wonder

被引：17

作者：

Bassil, Nahla V.

Njuguna, Wambui

Slovin, Janet P.

机构：

[1] USDA ARS, NCGR, Corvallis, OR 97333 USA

[2] Oregon State Univ, Dept Hort, Corvallis, OR 97333 USA

[3] USDA ARS, Beltsville Agr Res Ctr W, Fruit Lab, Beltsville, MD 20705 USA

来源：

MOLECULAR ECOLOGY NOTES | 2006年 / 6卷 / 03期

关键词：

microsatellite; strawberry;

D O I：

10.1111/j.1471-8286.2006.01351.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Fourteen microsatellite primer pairs were developed from a cDNA library of heat-treated seedlings of Fragaria vesca cv. yellow wonder. Transferability to 13 species of Fragaria ranged from 71% in diploid species F. gracilis Losinsk., F. iinumae Makino, F. nilgerrensis Schltdl. ex J. Gay and F. nipponica Makino, to 100% in octoploid domestic strawberry and its progenitors. Polymorphism was high in polyploid Fragaria species. However, polymorphism and heterozygosity of eight EST-SSRs (expressed sequence tag-simple sequence repeats) was low in 14 F. vesca genotypes.

引用

页码：806 / 809

页数：4

共 5 条

[1]

Hancock J.F, 1999, STRAWBERRIES

[2] Isolation and characterization of polymorphic microsatellites in diploid strawberry (Fragaria vesca L.) for mapping, diversity studies and clone identification [J].