Toxicity of a Bacillus thuringiensis israelensis-like strain against Spodoptera frugiperda

被引:6
作者
Antonino de Souza, Jose D., Jr. [2 ]
Jain, Sona [1 ]
Fontes de Oliveira, Claudia Maria [3 ]
Ayres, Constancia F. [3 ]
Lucena, Wagner Alexandre [1 ]
机构
[1] Embrapa Algodao, BR-58107720 Campina Grande, PB, Brazil
[2] Univ Fed Pernambuco, CCB, Dept Zool, BR-50670420 Recife, PE, Brazil
[3] CPqAM, Dept Entomol, BR-50670420 Recife, PE, Brazil
关键词
Bacillus thuringiensis; Biochemical characterization; delta-Endotoxins; Fall armyworm; Lepidoptera; Noctuidae; Molecular characterization; SUBSP ISRAELENSIS; CRY GENES; CULEX-QUINQUEFASCIATUS; LEPIDOPTERA; NOCTUIDAE; CRYSTAL; IDENTIFICATION; CYT1AA; PCR; COMPONENTS;
D O I
10.1007/s10526-008-9191-8
中图分类号
Q96 [昆虫学];
学科分类号
摘要
Bacillus thuringiensis (Bt) Berliner is a promising agent for microbial control of agriculturally and medically important insects. This study aimed at searching for Bt strains encoding Cry proteins that act more efficiently against fall armyworm. Thirty Bt strains were isolated from soil samples in Pernambuco State and evaluated through bioassays. Among these, strain I4A7 was the most efficient against the fall armyworm, Spodoptera frugiperda (J. E. Smith, 1797) (Lepidoptera: Noctuidae), and thus it was characterized by biochemical sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and molecular (polymerase chain reaction (PCR) and sequencing reaction) methods. The protein pattern of this strain on a SDS-PAGE was similar to that of B. thuringiensis israelensis (Bti). Moreover, I4A7 cry DNA sequence showed high identity (99-100%) to genes cry4Aa, 4Ba, 10Aa, 11Aa, cyt1Aa and cyt2B from Bti. The toxicity of the newly isolated Bti-like strain upon S. frugiperda should be considered as this strain might be used in combination with other Bt strains, such as B. thuringiensis var. kurstaki (Btk).
引用
收藏
页码:467 / 473
页数:7
相关论文
共 32 条
[1]   BASIC LOCAL ALIGNMENT SEARCH TOOL [J].
ALTSCHUL, SF ;
GISH, W ;
MILLER, W ;
MYERS, EW ;
LIPMAN, DJ .
JOURNAL OF MOLECULAR BIOLOGY, 1990, 215 (03) :403-410
[2]  
[Anonymous], 1986, CRIACAO INSETOS ESTU
[3]   Diversity of Bacillus thuringiensis strains from Colombia with insecticidal activity against Spodoptera frugiperda (Lepidoptera:Noctuidae) [J].
Arango, JA ;
Romero, M ;
Orduz, S .
JOURNAL OF APPLIED MICROBIOLOGY, 2002, 92 (03) :466-474
[4]   Cloning, sequencing, and expression of the chitinase gene chiA74 from Bacillus thuringiensis [J].
Barboza-Corona, JE ;
Nieto-Mazzocco, E ;
Veldázquez-Robledo, R ;
Salcedo-Hernandez, R ;
Bautista, M ;
Jiménez, B ;
Ibarra, JE .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2003, 69 (02) :1023-1029
[5]   Extended screening by PCR for seven cry-group genes from field-collected strains of Bacillus thuringiensis [J].
BenDov, E ;
Zaritsky, A ;
Dahan, E ;
Barak, Z ;
Sinai, R ;
Manasherob, R ;
Khamraev, A ;
Troitskaya, E ;
Dubitsky, A ;
Berezina, N ;
Margalith, Y .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1997, 63 (12) :4883-4890
[6]  
Bravo A, 1998, APPL ENVIRON MICROB, V64, P4965
[7]   Interaction of the delta-endotoxin CytA from Bacillus thuringiensis var. israelensis with lipid membranes [J].
Butko, P ;
Huang, F ;
PusztaiCarey, M ;
Surewicz, WK .
BIOCHEMISTRY, 1997, 36 (42) :12862-12868
[8]  
CRICKMORE N, 1995, FEMS MICROBIOL LETT, V131, P249
[9]  
Delecluse A., 2000, ENTOMOPATHOGENIC BAC, P101
[10]  
Federici BA, 1998, APPL ENVIRON MICROB, V64, P4368