An IS900-like sequence found in a Mycobacterium sp other than Mycobacterium avium subsp paratuberculosis

被引：148

作者：

Englund, S ^{[1
]}

Bölske, G ^{[1
]}

Johansson, KE ^{[1
]}

机构：

[1] Natl Vet Inst, Dept Bacteriol, SE-75189 Uppsala, Sweden

来源：

FEMS MICROBIOLOGY LETTERS | 2002年 / 209卷 / 02期

关键词：

diagnosis; IS900; polymerase chain reaction; Mycobacterium avium subsp; paratuberculosis;

D O I：

10.1111/j.1574-6968.2002.tb11142.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The insertion sequence IS900 has been considered specific for Mycobacterium avium subsp. paratuberculosis (M. paratuberculosis) and has, therefore, been used as the target gene for diagnostic PCR of M paratuberculosis. From a healthy dairy cow we have isolated and characterised a mycobacterium harbouring one copy of a sequence with 94% identity to IS900 at the nucleic acid level. The isolate was shown to be related to Mycobacterium cookil, as assessed by 16S rRNA sequencing. Strong amplifications were obtained with several PCR primers described for detection of IS900. This finding shows the need of alternative PCR systems based on other genes than IS900 to confirm the presence of M. paratuberculosis. (C) 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：267 / 271

页数：5

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