Notch Ligand Presenting Acellular 3D Microenvironments for ex vivo Human Hematopoietic Stem-Cell Culture made by Layer-By-Layer Assembly

被引:60
作者
Lee, Jungwoo [1 ,2 ,3 ]
Kotov, Nicholas A. [1 ,2 ,3 ]
机构
[1] Univ Michigan, Dept Biomed Engn, Ann Arbor, MI 48109 USA
[2] Univ Michigan, Dept Chem Engn, Ann Arbor, MI 48109 USA
[3] Univ Michigan, Dept Mat Sci & Engn, Ann Arbor, MI 48109 USA
关键词
layer-by-layer assembly; ligands; scaffolds; stem cells; thymus; COLLOIDAL-CRYSTAL GEOMETRY; DELTA-LIKE-1; IN-VITRO; PROGENITOR CELLS; BONE-MARROW; LBL FILMS; T-CELLS; SCAFFOLDS; PRECURSORS; GENERATION; INDUCTION;
D O I
10.1002/smll.200801242
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
An effort to combine 3D microarchitecture and notch ligand effects for in vitro hematopoietic stem cell (HSC) cultures under the hypothesis that 3D inverted colloidal crystal (ICC) scaffolds coated with DL-1 notch ligand would considerably substitute for the structure and function of thymic stroma was reported. A layer-by-layer (LBL) molecular assembly technique was utilized to immobilize DL-1 notch ligands on the surface of the ICC pores since the solution-based LBL coating procedure can be readily applied to the surface coating of complex 3D porous substrates while minimizing distortion of protein structures. The pre-deposited LBL film provides a better environment for the DL-1 notch ligand coating by forming higher electrostatic charges than the bare hydrogel surface. The cross-sectional image of an ICC scaffold after 15 days of culture shows that a significant number of cells residing in the inner pores of the scaffold.
引用
收藏
页码:1008 / 1013
页数:6
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