Coptisine suppresses proliferation and inhibits metastasis in human pancreatic cancer PANC-1 cells

被引：15
|
作者：
Ya-Li Zhang [1 ]
Zhang, Xiao [1 ]
Miao, Xiang-Zhen [1 ]
Yuan, Yan-Yan [1 ]
Gao, Jian [1 ]
Li, Xia [2 ]
Liu, Yong-Gang [1 ]
Tan, Peng [1 ]
机构：
[1] Beijing Univ Chinese Med, Sch Chinese Mat Mad, Beijing 100029, Peoples R China
[2] Chinese Acad Sci, Inst Genet & Dev Biol, Beijing 100101, Peoples R China
来源：
JOURNAL OF ASIAN NATURAL PRODUCTS RESEARCH | 2020年 / 22卷 / 05期
基金：
中国国家自然科学基金;
关键词：
Pancreatic cancer; coptisine; PANC-1; cell; PROTEIN-KINASE CASCADE; TUMOR-GROWTH; IN-VITRO; ADENOCARCINOMA; STATISTICS;
D O I：
10.1080/10286020.2019.1585820
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
The effects of coptisine against advanced stage of human pancreatic carcinoma PANC-1 cells was investigated in vitro. Coptisine (25-150 mu M) treatment for 48 h caused dose-dependent cell growth inhibition by using CCK-8 assay. Additionally, coptisine was found to inhibit PANC-1 cells metastasis by the wound healing assay. Flow cytometry data indicated that coptisine (25-100 mu M) exhibited dose-dependent G1 phase arrest and moderate reduction of S phase. Coptisine was also found to inhibit ERK phosphorylation and total ERK levels. Our research suggested that coptisine would be a potential therapeutic drug for the treatment of pancreatic cancer. [GRAPHICS] .
引用
收藏
页码：452 / 463
页数：12
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