3,3′,4,4′,5-Pentachlorobiphenyl influences mitochondrial apoptosis pathway in granulosa cells

被引：8

作者：

Zhong, Tao ^{[1
]}

Zhang, Jianmei ^{[1
]}

Han, Xiaoying ^{[1
]}

Gongye, Xiaoxiao ^{[1
]}

Lyu, Tianqi ^{[1
]}

Jiang, Menghan ^{[1
,2
]}

Yu, Kaiwei ^{[1
]}

Meng, Xiaoqian ^{[1
]}

Cheng, Dong ^{[3
]}

Lyu, Hui ^{[3
]}

Zhang, Tianliang ^{[3
]}

Zhang, Lei ^{[2
]}

Liu, Shuzhen ^{[1
]}

机构：

[1] Shandong Normal Univ, Coll Life Sci, Shandong Prov Key Lab Anim Resistance Biol, Jinan 250014, Shandong, Peoples R China

[2] Shandong Normal Univ, Coll Life Sci, CMC, Jinan, Shandong, Peoples R China

[3] Shandong Ctr Dis Control & Prevent, Dept Toxicol, Jinan, Shandong, Peoples R China

来源：

JOURNAL OF CELLULAR BIOCHEMISTRY | 2019年 / 120卷 / 09期

基金：

中国国家自然科学基金;

关键词：

apoptosis-related proteins; granulosa cells; mice; PCB126; POLYCHLORINATED-BIPHENYLS; ORGANOCHLORINE PESTICIDES; EMBRYO DEVELOPMENT; STEROID-SECRETION; PORCINE THECA; PCB; 126; EXPOSURE; OVARIES; IMPAIRS; OOCYTE;

D O I：

10.1002/jcb.28801

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

3,3 ',4,4 ',5-Polychlorinated biphenyl (PCB126) is a persistent organic environmental pollutant which can affect various biological activities of organisms, such as immunity, neurological function, and reproduction. In our study, we aimed to investigate the effects of PCB126 on granulosa cells (GCs). GCs were collected from ovaries in PMSG-treated mice, after 24 hours culture. GCs were then incubated with 10 pg/mL, 100 pg/mL, and 10 ng/mL of PCB126 for another 24 hours. Following these steps, exposed GCs were collected for further experimentation. Our data showed that the number of GCs in the 10 ng/mL PCB126 decreased. Meanwhile, pyknotic nuclei and condensed chromatin increased, while the apoptotic cells in the 10 ng/mL PCB126 group were significantly increased. Furthermore, the expression of the apoptotic executive protein caspase-3 increased after PCB126 treatment. The expression of Bax, Bcl-2, and Bim related to the mitochondrial apoptosis pathway were also influenced to different degrees. Thus, our data suggested that PCB126 affect the GCs apoptosis, and mitochondrial apoptosis pathway was involved in this process.

引用

页码：15337 / 15346

页数：10

共 45 条

[1] Polychlorinated biphenyls (PCBs) alter DNA methylation and genomic integrity of sheep fetal cells in a simplified in vitro model of pregnancy exposure [J].