Role of bacterial pyrroloquinoline quinone in phosphate solubilizing ability and in plant growth promotion on strain Serratia sp S119

被引:26
作者
Mercedes Luduena, Liliana [1 ]
Soledad Anzuay, Maria [1 ]
Guillermo Angelini, Jorge [1 ]
Barros, German [2 ]
Flavia Luna, Maria [3 ]
del Pilar Monge, Maria [2 ]
Fabra, Adriana [1 ]
Taurian, Tania [1 ]
机构
[1] Univ Nacl Rio Cuarto, Fac Ciencias Exactas Fis Quim & Nat, Dept Ciencias Nat, Agencia Postal 3, RA-5800 Cordoba, Argentina
[2] Univ Nacl Rio Cuarto, Fac Ciencias Exactas Fis Quim & Nat, Dept Microbiol, Agencia Postal 3, RA-5800 Cordoba, Argentina
[3] Univ Nacl La Plata, Ctr Invest & Desarrollo Fermentac Ind CONICET, RA-1900 Buenos Aires, DF, Argentina
关键词
PQQ cofactor; Serratia sp; Phosphate solubilization; Plant growth promotion; pqqE sequence; NUCLEOTIDE-SEQUENCE; SOIL; BIOSYNTHESIS; GENES; MICROORGANISMS; MECHANISMS; RHIZOBIUM; GENETICS; CLONING; GLUCOSE;
D O I
10.1007/s13199-016-0434-7
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The aim of this study was to analyze if cofactor pyrroquinoline quinone from Serratia sp. S119 is involved in the inorganic phosphate solubilization mechanism and in its ability to promote the plant growth. Site directed mutagenesis was performed to obtain a pqqE- minus mutant of strain Serratia sp. S119. The phosphate solubilization ability, gluconate and PQQ production of the mutant Serratia sp. RSL (pqqE-) was analyzed. Mutant RSL (pqqE-) showed significant decrease in P soluble and gluconic acid levels produced and undetectable levels of PQQ cofactor compared with wild-type strain. Complementation with synthetic PQQ cofactor restored P solubilization and gluconate production reaching the levels produced by wild-type strain. PqqE gene sequence indicated that it is highly conserved within Serratia strains and its product shows conserved motifs found in other PqqE proteins of several bacteria. The effect of the inoculation of the PQQ- mutant on peanut and maize plants was evaluated in pot assays. Plants growth parameters showed no differences among the different treatments indicating that PQQ from Serratia sp. S119 is not involved in the growth promotion of these plants. PQQ cofactor is essential for phosphate solubilization ability of Serratia sp. S119 but is not required for growth promotion of peanut and maize plants.
引用
收藏
页码:31 / 43
页数:13
相关论文
共 57 条
[1]   The pKNOCK series of broad-host-range mobilizable suicide vectors for gene knockout and targeted DNA insertion into the chromosome of Gram-negative bacteria [J].
Alexeyev, MF .
BIOTECHNIQUES, 1999, 26 (05) :824-+
[2]   The quinoprotein dehydrogenases for methanol and glucose [J].
Anthony, C .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 2004, 428 (01) :2-9
[3]   Plant- and microbial-based mechanisms to improve the agronomic effectiveness of phosphate rock: a review [J].
Arcand, Melissa M. ;
Schneider, Kim D. .
ANAIS DA ACADEMIA BRASILEIRA DE CIENCIAS, 2006, 78 (04) :791-807
[4]  
Ausubel F. M., 2003, Current protocols in molecular biology
[5]  
BIVILLE F, 1989, J GEN MICROBIOL, V135, P2917
[6]   Phosphate solubilizing bacteria from subtropical soil and their tricalcium phosphate solubilizing abilities [J].
Chen, Y. P. ;
Rekha, P. D. ;
Arun, A. B. ;
Shen, F. T. ;
Lai, W. -A. ;
Young, C. C. .
APPLIED SOIL ECOLOGY, 2006, 34 (01) :33-41
[7]   Pyrroloquinoline quinone is a plant growth promotion factor produced by Pseudomonas fluorescens B16 [J].
Choi, Okhee ;
Kim, Jinwoo ;
Kim, Jung-Gun ;
Jeong, Yeonhwa ;
Moon, Jae Sun ;
Park, Chang Seuk ;
Hwang, Ingyu .
PLANT PHYSIOLOGY, 2008, 146 (02) :657-668
[8]   USE OF REPETITIVE (REPETITIVE EXTRAGENIC PALINDROMIC AND ENTEROBACTERIAL REPETITIVE INTERGENERIC CONSENSUS) SEQUENCES AND THE POLYMERASE CHAIN-REACTION TO FINGERPRINT THE GENOMES OF RHIZOBIUM-MELILOTI ISOLATES AND OTHER SOIL BACTERIA [J].
DEBRUIJN, FJ .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1992, 58 (07) :2180-2187
[9]   Serratia marcescens Quinoprotein Glucose Dehydrogenase Activity Mediates Medium Acidification and Inhibition of Prodigiosin Production by Glucose [J].
Fender, James E. ;
Bender, Cody M. ;
Stella, Nicholas A. ;
Lahr, Roni M. ;
Kalivoda, Eric J. ;
Shanks, Robert M. Q. .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2012, 78 (17) :6225-6235
[10]  
Fiske E. H., 1925, NEW J SCI TECHNOL, V33, P436