Expression of γ-H2AX, 53BP1 and Micronuclei as Genome Damage Biomarker of Population in Keang and Salumati Village, Mamuju West Sulawesi Province

被引：0

作者：

Pratiwi, A. D. ^{[1
]}

Basri, I. K. H. ^{[2
]}

Purnami, S. ^{[2
]}

Syaifudin, M. ^{[2
]}

Tetriana, D. ^{[2
]}

Yusuf, D. ^{[2
]}

Rahardjo, T. ^{[2
]}

Nurhayati, S. ^{[2
]}

Rahajeng, N. ^{[2
]}

Anggraito, Y. U. ^{[1
]}

Kashiwakura, I. ^{[3
]}

机构：

[1] Semarang State Univ, Fac Math & Sci, Dept Biol, Kampus Sekaran, Gunungpati 50229, Semarang, Indonesia

[2] Natl Nucl Energy Agcy, Ctr Radiat Safety Technol & Metrol, Jl Lebak Bulus Raya 49, Jakarta 12440, Indonesia

[3] Hirosaki Univ, Grad Sch Hlth Sci, Dept Radiol Life Sci, 66-1 Hon Cho, Hirosaki, Aomori 036856, Japan

来源：

ATOM INDONESIA | 2019年 / 45卷 / 02期

关键词：

gamma-H2AX; 53BP1; DNA damage; Exposed area; Control area; PERIPHERAL-BLOOD LYMPHOCYTES; BACKGROUND-RADIATION AREAS; IONIZING-RADIATION; DNA-DAMAGE; DICENTRIC CHROMOSOME; ADAPTIVE RESPONSE; LIFE-STYLE; CANCER; ASSAY; AGE;

D O I：

10.17146/aij.2019.847

中图分类号：

TL [原子能技术]; O571 [原子核物理学];

学科分类号：

0827 ; 082701 ;

摘要：

The residents living in high background radiation area have risk to be exposed by ionizing radiation that also potentially cause their DNA damage. The aim of this study was to determine the expression of gamma-H2AX, 53BP1 foci and micronuclei in the residents who live in high background radiation area of Salumati village, Mamuju, West Sulawesi, Indonesia. Twenty one blood samples which consist of 11 from the study area and 10 from control were assessed for their expression of gamma-H2AX and 53BPI foci by using specific antibodies and observed under fluorescence microscope whereas micronuclei was detected after being cultured and giemsa stained according to standard procedures. Results showed that both gamma-H2AX and 53BP1foci from high background area was lower than that of control area (0.37 +/- 0.24 vs 0.19 +/- 0.11 (p=0.03) for gamma-H2AX and 0.61 +/- 0.30 vs 0.31 +/- 0.12 for 53BP1 (p=0.01)). The mean of micronuclei frequency in exposed area was 0.02 (0,01-0,03) while in control area was 0.02 (0.003-0.02). There was statistical significant in corellation between both gamma-H2AX, 53BP1 foci with micronuclei index in exposed area (p= 0.02, p= 0,04 respectively). In conclusion, there was a positive correlation between gamma-H2AX and 53BP1 foci to micronuclei and this might be a clue of the occurrence of genome repairing mechanism caused by natural radiation at low dose chronical exposure in the studied area. (C) 2019 Atom Indonesia. All rights reserved

引用

页码：103 / 108

页数：6

共 31 条

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