Label-Free Detection of Biomolecular Interactions Using BioLayer Interferometry for Kinetic Characterization

被引：261

作者：

Concepcion, Joy ^{[1
]}

Witte, Krista ^{[1
]}

Wartchow, Charles ^{[1
]}

Choo, Sae ^{[1
]}

Yao, Danfeng ^{[1
]}

Persson, Henrik ^{[1
]}

Wei, Jing ^{[1
]}

Li, Pu ^{[1
]}

Heidecker, Bettina ^{[1
]}

Ma, Weilei ^{[1
]}

Varma, Ram ^{[1
]}

Zhao, Lian-She ^{[1
]}

Perillat, Donald ^{[1
]}

Carricato, Greg ^{[1
]}

Recknor, Michael ^{[1
]}

Du, Kevin ^{[1
]}

Ho, Huddee ^{[1
]}

Ellis, Tim ^{[1
]}

Gamez, Juan ^{[1
]}

Howes, Michael ^{[1
]}

Phi-Wilson, Janette ^{[1
]}

Lockard, Scott ^{[1
]}

Zuk, Robert ^{[1
]}

Tan, Hong ^{[1
]}

机构：

[1] ForteBio Inc, Menlo Pk, CA 94025 USA

来源：

COMBINATORIAL CHEMISTRY & HIGH THROUGHPUT SCREENING | 2009年 / 12卷 / 08期

关键词：

SURFACE-PLASMON RESONANCE; BIACORE TECHNOLOGY; SENSORS; BINDING; LIGANDS; DNA;

D O I：

10.2174/138620709789104915

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The analysis of biomolecular interactions is key in the drug development process. Label-free biosensor methods provide information on binding, kinetics, concentration, and the affinity of an interaction. These techniques provide real-time monitoring of interactions between an immobilized ligand (such as a receptor) to an analyte in solution without the use of labels. Advances in biosensor design and detection using BioLayer Interferometry (BLI) provide a simple platform that enables label-free monitoring of biomolecular interactions without the use of flow cells. We review the applications of BLI in a wide variety of research and development environments for quantifying antibodies and proteins and measuring kinetics parameters.

引用

页码：791 / 800

页数：10

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