Expression of Talin1 in tissues of ovarian cancer and its role in invasion and migration of ovarian cancer cells

被引：0

作者：

Wang, Jun ^{[1
]}

Zhou, Heling ^{[1
]}

Wang, Kai ^{[1
]}

Shang, Xianwen ^{[1
]}

Chen, Xing ^{[1
]}

Zheng, Lingzhi ^{[1
]}

Yu, Junhui ^{[1
]}

机构：

[1] Tai Zhou Hosp Zhe Jiang Prov, Dept Gynecol, Xi Men Ave 150, Lin Hai, Zhejiang, Peoples R China

来源：

INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE | 2017年 / 10卷 / 01期

关键词：

Talin1; ovarian cancer; EMT; E-Cadherin; transwell; IDENTIFICATION; METASTASIS;

D O I：

暂无

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Objective: To investigate the expression of Talin1 in tissues of ovarian cancer and its role in invasion and migration of ovarian cancer cells as well as the related mechanisms. Method: The expression of Talin1 in the tissues of ovarian cancer and normal ovarian tissues was detected by immunohistochemical methods; lentivirus silencing Talin1, GFP fluorescence and Western blotting were used to detect the LV3-Talin1 silencing efficiency and efficacy; the effect of Talin1 expression on invasion of ovarian cancer cells was detected by Transwell invasion assay; and the effect of Talin1 expression on migration of ovarian cancer cells was detected by wound scratch test; and expression of the proteins E-Cadherin, Vimentin and Twist was detected by Western blotting. Results: Compared with normal ovarian tissues, the Talin1 expression in tissues of ovarian cancer was significantly increased; and the Talin1 expression in tissues of advanced, poorly differentiated ovarian cancer with lymph node metastasis was significantly higher than that in tissues of early, well-differentiated ovarian cancer without lymph node metastasis; LV3-Talin1 lentivirus could effectively inhibit the expression of Talin1; Talin1 silencing could inhibit invasion and migration of the A2780 cells; Talin1 silencing up-regulated the expression of E-Cadherin and down-regulated the expression of Vimentin and Twist. Conclusion: Talin1 expression is significantly increased in ovarian cancer, and Talin1 is closely associated with staging and grading of ovarian cancer; in addition, Talin1 silencing promotes invasion and migration of ovarian cancer cells by inhibiting epithelial-mesenchymal transition (EMT).

引用

页码：410 / 417

页数：8

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