Fabrication of PHBV/Keratin Composite Nanofibrous Mats for Biomedical Applications

被引：67

作者：

Yuan, Jiang ^{[1
,2
]}

Xing, Zhi-Cai ^{[1
]}

Park, Suk-Woo ^{[1
]}

Geng, Jia ^{[1
]}

Kang, Inn-Kyu ^{[1
]}

Yuan, Jiang ^{[1
,2
]}

Shen, Jian ^{[2
]}

Meng, Wan ^{[3
]}

Shim, Kyoung-Jin ^{[4
]}

Han, In-Suk ^{[4
]}

Kim, Jung-Chul ^{[4
]}

机构：

[1] Kyungpook Natl Univ, Dept Polymer Sci & Engn, Taegu 702701, South Korea

[2] Nanjing Normal Univ, Jiangsu Engn Res Ctr Biomed Funct Mat, Nanjing 210097, Peoples R China

[3] Yanbian Univ, Dept Chem Engn & Polymer Sci, Yanji 133002, Peoples R China

[4] Kyungpook Natl Univ, Dept Immunol, Sch Med, Taegu 702701, South Korea

来源：

MACROMOLECULAR RESEARCH | 2009年 / 17卷 / 11期

关键词：

biomedical; fibrobalsts; keratin; nanofiber; PHBV; CARBOXYMETHYLATED FEATHER KERATINS; POLYMER THIN-FILMS; COLLAGEN NANOFIBERS; SURFACE MODIFICATION; SOLUBLE DERIVATIVES; ACID-SOLUTION; CHITOSAN; CULTIVATION; FIBERS;

D O I：

10.1007/BF03218625

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

Keratin is an important protein used in wound healing and tissue recovery. In this study, keratin was modified chemically with iodoacetic acid (IAA) to enhance its solubility in organic solvent. Poly(hydroxybutylate-co-hydroxyvalerate) (PHBV) and modified keratin were dissolved in hexafluoroisopropanol (HFIP) and electrospun to produce nanofibrous mats. The resulting mats were surface-characterized by ATR-FTIR, field-emission scanning electron microscopy (FE-SEM) and electron spectroscopy for chemical analysis (ESCA). The pure m-keratin mat was cross-linked with glutaraldehyde vapor to make it insoluble in water. The biodegradation test in vitro showed that the mats could be biodegraded by PHB depolymerase and trypsin aqueous solution. The results of the cell adhesion experiment showed that the NIH 3T3 cells adhered more to the PHBV/m-keratin nanofibrous mats than the PHBV film. The BrdU assay showed that the keratin and PHBV/m-keratin nanofibrous mats could accelerate the proliferation of fibroblast cells compared to the PHBV nanofibrous mats.

引用

页码：850 / 855

页数：6

共 37 条

[1] Electrospun chitosan-based nanofibers and their cellular compatibility [J].

Bhattarai, N ;

Edmondson, D ;

Veiseh, O ;

Matsen, FA ;

Zhang, MQ .

BIOMATERIALS, 2005, 26 (31) :6176-6184

[2] Surface characterization of porous, biocompatible protein polymer thin films [J].

Buchko, CJ ;

Kozloff, KM ;

Martin, DC .

BIOMATERIALS, 2001, 22 (11) :1289-1300

[3] Processing and microstructural characterization of porous biocompatible protein polymer thin films [J].

Buchko, CJ ;

Chen, LC ;

Shen, Y ;

Martin, DC .

POLYMER, 1999, 40 (26) :7397-7407

[4]

CLELAND JL, 1993, CRIT REV THER DRUG, V10, P307

[5] Biomimetic surface modification of poly(L-lactic acid) with chitosan and its effects on articular chondrocytes in vitro [J].

Cui, YL ;

Di Qi, A ;

Liu, WG ;

Wang, XH ;

Wang, H ;

Ma, DM ;

De Yao, K .

BIOMATERIALS, 2003, 24 (21) :3859-3868

[6] A COLORIMETRIC METHOD FOR DETERMINING LOW CONCENTRATIONS OF MERCAPTANS [J].

ELLMAN, GL .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1958, 74 (02) :443-450

[7] PHYSICAL-PROPERTIES OF POLY(BETA-HYDROXYBUTYRATE) POLY(EPSILON-CAPROLACTONE) BLENDS [J].

GASSNER, F ;

OWEN, AJ .

POLYMER, 1994, 35 (10) :2233-2236

[8] Electrospinning of chitosan dissolved in concentrated acetic acid solution [J].

Geng, XY ;

Kwon, OH ;

Jang, JH .

BIOMATERIALS, 2005, 26 (27) :5427-5432

[9] SOLUBLE DERIVATIVES OF FEATHER KERATIN .2. MOLECULAR WEIGHT + CONFORMATION [J].

HARRAP, BS ;

WOODS, EF .

BIOCHEMICAL JOURNAL, 1964, 92 (01) :19-&

[10] SOLUBLE DERIVATIVES OF FEATHER KERATIN .1. ISOLATION FRACTIONATION + AMINO ACID COMPOSITION [J].

HARRAP, BS ;

WOODS, EF .

BIOCHEMICAL JOURNAL, 1964, 92 (01) :8-+

← 1 2 3 4 →