Overexpression of miR-224-3p alleviates apoptosis from cerebral ischemia reperfusion injury by targeting FIP200

被引：36

作者：

Deng, Yiming ^{[1
,2
,3
]}

Ma, Gaoting ^{[1
,2
,3
]}

Dong, Qihao ^{[4
]}

Sun, Xuan ^{[1
,2
,3
]}

Liu, Lian ^{[1
,2
,3
]}

Miao, Zhongrong ^{[1
,2
,3
]}

Gao, Feng ^{[1
,2
,3
]}

机构：

[1] Capital Med Univ, Beijing Tiantan Hosp, Dept Intervent Neuroradiol, Beijing, Peoples R China

[2] China Natl Clin Res Ctr Neurol Dis, Beijing, Peoples R China

[3] Beijing Inst Brain Disorders, Ctr Stroke, Beijing, Peoples R China

[4] Cent Hosp Zibo, Dept Neurol, Zibo, Peoples R China

来源：

JOURNAL OF CELLULAR BIOCHEMISTRY | 2019年 / 120卷 / 10期

关键词：

apoptosis; FIP200; ischemic stroke; miR-224-3p; EXPRESSION; THROMBECTOMY; MICRORNAS; CANCER; STROKE;

D O I：

10.1002/jcb.28975

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Aims In previous studies, numerous differential microRNAs (miRNAs) in cerebral ischemic/reperfusion (I/R) injury were identified using the miRNA microarray analysis. However, the relationship between miRNA and cerebral I/R injury remains largely unknown. In this study, we investigated the function and explored the possible mechanism of miR-224-3p in cerebral I/R injury. Methods Oxygen glucose deprivation model in N2a cells were used to perform the cerebral I/R injury in vitro. Trypan blue staining, reactive oxygen species (ROS) production, and caspase-3 were measured to evaluate the function of miR-224-3p. Results Overexpression of miR-224-3p alleviated the apoptosis induced by oxygen glucose deprivation (OGD) and cleaved caspase-3 was significantly reduced. We further provided the possible mechanism that miR-224-3p may protect cells from cerebral I/R injury by targeting FAK family-interacting protein (FIP200). Further rescue experiment proved that overexpression of FIP200 partially blocked the effect of miR-224-3p. Conclusions We evaluated the function and mechanism of miR-224-3p in ischemic brain injury. miR-224-3p may serve as a potential target for new therapeutic intervention.

引用

页码：17151 / 17158

页数：8

共 20 条

[1] A microRNA screen reveals that elevated hepatic ectodysplasin A expression contributes to obesity-induced insulin resistance in skeletal muscle [J].

Awazawa, Motoharu ;

Gabel, Paula ;

Tsaousidou, Eva ;

Nolte, Hendrik ;

Krueger, Marcus ;

Schmitz, Joel ;

Ackermann, P. Justus ;

Brandt, Claus ;

Altmueller, Janine ;

Motameny, Susanne ;

Wunderlich, F. Thomas ;

Kornfeld, Jan-Wilhelm ;

Blueher, Matthias ;

Bruning, Jens C. .

NATURE MEDICINE, 2017, 23 (12) :1466-+

[2] The emerging roles of microRNAs in CNS injuries [J].

Bhalala, Oneil G. ;

Srikanth, Maya ;

Kessler, John A. .

NATURE REVIEWS NEUROLOGY, 2013, 9 (06) :328-339

[3] Tenecteplase versus Alteplase before Thrombectomy for Ischemic Stroke [J].

Campbell, B. C. V. ;

Mitchell, P. J. ;

Churilov, L. ;

Yassi, N. ;

Kleinig, T. J. ;

Dowling, R. J. ;

Yan, B. ;

Bush, S. J. ;

Dewey, H. M. ;

Thijs, V. ;

Scroop, R. ;

Simpson, M. ;

Brooks, M. ;

Asadi, H. ;

Wu, T. Y. ;

Shah, D. G. ;

Wijeratne, T. ;

Ang, T. ;

Miteff, F. ;

Levi, C. R. ;

Rodrigues, E. ;

Zhao, H. ;

Salvaris, P. ;

Garcia-Esperon, C. ;

Bailey, P. ;

Rice, H. ;

de Villiers, L. ;

Brown, H. ;

Redmond, K. ;

Leggett, D. ;

Fink, J. N. ;

Collecutt, W. ;

Wong, A. A. ;

Muller, C. ;

Coulthard, A. ;

Mitchell, K. ;

Clouston, J. ;

Mahady, K. ;

Field, D. ;

Ma, H. ;

Phan, T. G. ;

Chong, W. ;

Chandra, R. V. ;

Slater, L. -A. ;

Krause, M. ;

Harrington, T. J. ;

Faulder, K. C. ;

Steinfort, B. S. ;

Bladin, C. F. ;

Sharma, G. .

NEW ENGLAND JOURNAL OF MEDICINE, 2018, 378 (17) :1573-1582

[4] Endovascular Therapy for Ischemic Stroke with Perfusion-Imaging Selection [J].

Campbell, B. C. V. ;

Mitchell, P. J. ;

Kleinig, T. J. ;

Dewey, H. M. ;

Churilov, L. ;

Yassi, N. ;

Yan, B. ;

Dowling, R. J. ;

Parsons, M. W. ;

Oxley, T. J. ;

Wu, T. Y. ;

Brooks, M. ;

Simpson, M. A. ;

Miteff, F. ;

Levi, C. R. ;

Krause, M. ;

Harrington, T. J. ;

Faulder, K. C. ;

Steinfort, B. S. ;

Priglinger, M. ;

Ang, T. ;

Scroop, R. ;

Barber, P. A. ;

McGuinness, B. ;

Wijeratne, T. ;

Phan, T. G. ;

Chong, W. ;

Chandra, R. V. ;

Bladin, C. F. ;

Badve, M. ;

Rice, H. ;

de Villiers, L. ;

Ma, H. ;

Desmond, P. M. ;

Donnan, G. A. ;

Davis, S. M. .

NEW ENGLAND JOURNAL OF MEDICINE, 2015, 372 (11) :1009-1018

[5] Inhibition of miR-29c promotes proliferation, and inhibits apoptosis and differentiation in P19 embryonic carcinoma cells [J].

Chen, Bin ;

Song, Guixian ;

Liu, Ming ;

Qian, Lingmei ;

Wang, Lihua ;

Gu, Haitao ;

Shen, Yahui .

MOLECULAR MEDICINE REPORTS, 2016, 13 (03) :2527-2535

[6] MiRNA-29c regulates the expression of inflammatory cytokines in diabetic nephropathy by targeting tristetraprolin [J].

Guo, Jia ;

Li, Jing ;

Zhao, Jing ;

Yang, Shuguang ;

Wang, Luyao ;

Cheng, Genyang ;

Liu, Dong ;

Xiao, Jing ;

Liu, Zhangsuo ;

Zhao, Zhanzheng .

SCIENTIFIC REPORTS, 2017, 7

[7] MiR224-3p inhibits hypoxia-induced autophagy by targeting autophagy-related genes in human glioblastoma cells [J].

Guo, Xing ;

Xue, Hao ;

Guo, Xiaofan ;

Gao, Xiao ;

Xu, Shugang ;

Yan, Shaofeng ;

Han, Xiao ;

Li, Tong ;

Shen, Jie ;

Li, Gang .

ONCOTARGET, 2015, 6 (39) :41620-41637

[8] Thrombectomy within 8 Hours after Symptom Onset in Ischemic Stroke [J].

Jovin, T. G. ;

Chamorro, A. ;

Cobo, E. ;

de Miquel, M. A. ;

Molina, C. A. ;

Rovira, A. ;

San Roman, L. ;

Serena, J. ;

Abilleira, S. ;

Ribo, M. ;

Millan, M. ;

Urra, X. ;

Cardona, P. ;

Lopez-Cancio, E. ;

Tomasello, A. ;

Castano, C. ;

Blasco, J. ;

Aja, L. ;

Dorado, L. ;

Quesada, H. ;

Rubiera, M. ;

Hernandez-Perez, M. ;

Goyal, M. ;

Demchuk, A. M. ;

von Kummer, R. ;

Gallofre, M. ;

Davalos, A. .

NEW ENGLAND JOURNAL OF MEDICINE, 2015, 372 (24) :2296-2306

[9]

Kim KM, 2017, J HEPATOL, V03, P68

[10] Identification of Circulating MicroRNAs as Potential Biomarkers for Detecting Acute Ischemic Stroke [J].

Li, Pengfei ;

Teng, Fengmeng ;

Gao, Feng ;

Zhang, Mingshun ;

Wu, Jinping ;

Zhang, Chunbing .

CELLULAR AND MOLECULAR NEUROBIOLOGY, 2015, 35 (03) :433-447

← 1 2 →