Modulation of the resorption and osteoconductivity of α-calcium sulfate by histone deacetylase inhibitors

被引:36
作者
Jung, Hong-Moon [1 ,2 ]
Song, Gin-Ah [1 ,2 ]
Lee, Yong-Keun [2 ,3 ]
Baek, Jeong-Hwa [1 ,2 ]
Ryoo, Hyun-Mo [1 ,2 ]
Kim, Gwan-Shik [1 ,2 ]
Choung, Phil-Hoon [4 ]
Woo, Kyung Mi [1 ,2 ]
机构
[1] Seoul Natl Univ, Sch Dent, Dent Res Inst, Dept Cell & Dev Biol, Seoul 110749, South Korea
[2] Seoul Natl Univ, Sch Dent, Program BK21, Seoul 110749, South Korea
[3] Seoul Natl Univ, Sch Dent, Dent Res Inst, Dept Dent Biomat Sci, Seoul 110749, South Korea
[4] Seoul Natl Univ, Sch Dent, Tooth Bioengn Natl Res Lab, Dept Oral & Maxillofacial Surg, Seoul 110749, South Korea
关键词
Alpha calcium sulfate; Histone deacetylase inhibitors; Bone regeneration; Osteoblasts; Osteoclasts; MESENCHYMAL STEM-CELLS; TRICHOSTATIN-A; BONE; DIFFERENTIATION; HYDROXYAPATITE; OSTEOCLASTS; COMPOSITE; APOPTOSIS; CEMENTS; PLASTER;
D O I
10.1016/j.biomaterials.2009.09.019
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Calcium sulfate (CS) is an osteoconductive material with a long history of clinical use. However, its resorptive properties are not optimal for bone regeneration. Recently, histone deacetylase inhibitors (HDIs) have been suggested as bone regeneration tools. In this study. we investigated the effects of the HDIs sodium butyrate and trichostatin A on alpha-form CS (alpha CS) performance. MC3T3-E1 pre-osteoblasts cultured on alpha CS containing either HDI (alpha CS/HDI) showed higher levels of alkaline phosphatase activity than those cultured on alpha CS alone. The expression of genes characteristic of the osteoblast phenotype, including Runx2, osteocalcin, and bone sialoprotein, was strongly promoted by alpha CS/HDI. When cultured on alpha CS/HDIs, the osteoclastic differentiation of RAW264.7 monocytes was substantially suppressed, as measured by tartrate-resistant acid phosphatase (TRAP) activity and the expression levels of calcitonin receptor and TRAP. Neither HDI affected the CS setting time, compressive strength, or dissolution in a simulated body fluid. In a rat calvarial model of critical size bone defects, alpha CS/HDIs enhanced osteoblast differentiation, led to new bone formation, and delayed resorption, as confirmed by micro-computed tomography and histological analyses. (C) 2009 Elsevier Ltd. All rights reserved.
引用
收藏
页码:29 / 37
页数:9
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