Therapeutic drug monitoring of seven psychotropic drugs and four metabolites in human plasma by HPLC-MS

被引:108
作者
Choong, Eva [1 ]
Rudaz, Serge [2 ]
Kottelat, Astrid [1 ]
Guillarme, Davy [2 ]
Veuthey, Jean-Luc [2 ]
Eap, Chin B. [1 ,2 ]
机构
[1] Univ Lausanne, Hop Cery, Unit Biochem & Clin Psychopharmacol, Ctr Psychiat Neurosci,Dept Psychiat,CHUV, CH-1008 Prilly, Switzerland
[2] Univ Lausanne, Sch Pharmaceut Sci, Univ Geneva, CH-1211 Geneva 4, Switzerland
基金
新加坡国家研究基金会;
关键词
Method development; Quantification; Atypical antipsychotics; Antidepressants; SPE; LC-MS; Validation; TANDEM MASS-SPECTROMETRY; SOLID-PHASE EXTRACTION; LIQUID-CHROMATOGRAPHIC METHOD; BIOANALYTICAL METHODS VALIDATION; QUANTITATIVE-DETERMINATION; ATYPICAL ANTIPSYCHOTICS; ULTRAVIOLET DETECTION; ACTIVE METABOLITES; LC-MS; SERUM;
D O I
10.1016/j.jpba.2009.07.007
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A simple and sensitive LC-MS method was developed and validated for the simultaneous quantification of aripiprazole (ARI), atomoxetine (ATO), duloxetine (DUL), clozapine (CLO), olanzapine (OLA), sertindole (STN), venlafaxine (VEN) and their active metabolites dehydroaripiprazole (DARI), norclozapine (NCLO), dehydrosertindole (DSTN) and O-desmethylvenlafaxine (OVEN) in human plasma. The above mentioned compounds and the internal standard (remoxipride) were extracted from 0.5 mL plasma by solid-phase extraction (mix mode support). The analytical separation was carried out on a reverse phase liquid chromatography at basic pH (pH 8.1) in gradient mode. All analytes were monitored by MS detection in the single ion monitoring mode and the method was validated covering the corresponding therapeutic range: 2-200 ng/mL for DUI, OLA, and STN, 4-200 ng/mL for DSTN, 5-1000 ng/mL for ARI, DARI and finally 2-1000 ng/mL for ATO, CLO, NCLO, VEN, OVEN. For all investigated compounds, good performance in terms of recoveries, selectivity, stability, repeatability, intermediate precision, trueness and accuracy, was obtained. Real patient plasma samples were then successfully analysed. (C) 2009 Published by Elsevier B.V.
引用
收藏
页码:1000 / 1008
页数:9
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