Roundup® causes oxidative stress in liver and inhibits acetylcholinesterase in muscle and brain of the fish Prochilodus lineatus

This work aimed to evaluate Roundup (R) effects on biochemical biomarkers of the neotropical fish Prochilodus lineatus. Fish were acutely exposed (6, 24 and 96 h) to 10 mg L-1 of Roundup (R) (RD) or only water (control) and samples of liver, for antioxidants analysis, and brain and muscle, for acetylcholinesterase (AChE) determination, were collected. Fish exposed to RD for 24 h showed reduction on superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities, and increased glutathione (GSH) content. After 24 and 96 h, fish of RD group showed increased glutathione-S-transferase (GST) activity and lipid peroxidation. AChE activity was inhibited in brain after 96 h and in muscle after 24 and 96 h of exposure. Thus, acute exposure to RD stimulated the biotransformation pathway, with increased GST, but interfered on the antioxidant defenses, with reduction of SOD and GPx activity, leading to the occurrence of lipid peroxidation oxidation. Inhibition of AChE showed that RD acts as a contaminant with anti-AChE action. (C) 2009 Elsevier Ltd. All rights reserved.