Development of a Rapid Loop-Mediated Isothermal Amplification Assay for the Detection of Dothistroma septosporum

被引：12

作者：

Myrholm, Colin L. ^{[1
]}

Tomm, Bradley D. ^{[1
]}

Heinzelmann, Renate ^{[2
]}

Feau, Nicolas ^{[2
]}

Hamelin, Richard C. ^{[2
]}

McDougal, Rebecca ^{[3
]}

Winkworth, Richard C. ^{[4
]}

Ramsfield, Tod D. ^{[1
]}

机构：

[1] Northern Forestry Ctr, Canadian Forest Serv, Nat Resources Canada, 5320-122 St NW, Edmonton, AB T6H 3S5, Canada

[2] Univ British Columbia, Forest & Conservat Sci, Vancouver, BC V6T 1Z4, Canada

[3] Scion, Rotorua 3020, New Zealand

[4] Massey Univ, Sch Fundamental Sci, Palmerston North 4410, New Zealand

来源：

FORESTS | 2021年 / 12卷 / 03期

关键词：

DNA-based diagnostics; LAMP; Dothistroma needle blight;

D O I：

10.3390/f12030362

中图分类号：

S7 [林业];

学科分类号：

0829 ; 0907 ;

摘要：

A Loop-Mediated Isothermal Amplification (LAMP) assay was developed for the detection of the pine pathogen Dothistroma septosporum (G. Dorog.) M. Morelet. The specificity of the LAMP assay was tested using a selection of pine needle fungi, including Dothistroma pini Hulbary, and Lecanosticta acicola (Thum.) Syd.; only D. septosporum DNA was amplified by the test. In terms of sensitivity, the assay was able to detect as little as 1 pg of total D. septosporum DNA. This assay enables DNA extracted from diseased host needles to be rapidly tested for the presence of D. septosporum using relatively simple to operate equipment away from a fully equipped molecular biology laboratory.

引用

页数：10

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