Multicolour whole-mount in situ hybridization to Drosophila embryos

被引：20

作者：

Hauptmann, G

Gerster, T

机构：

[1] Biozentrum der Univ. Basel, Abteilung Zellbiologie, CH-4056 Basel

来源：

DEVELOPMENT GENES AND EVOLUTION | 1996年 / 206卷 / 04期

关键词：

biotin-UTP; alkaline phosphatase substrates; Fast Red; Fast Blue B; magenta-phos/INT;

D O I：

10.1007/s004270050055

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We report an extended whole-mount in situ hybridization procedure for Drosophila embryos. By using probes labelled with digoxigenin, fluorescein and biotin, respectively, this protocol allows the detection in three colours of RNAs derived from three different genes. Hybridized probes are detected by consecutive staining with appropriate alkaline phosphatase conjugates using different chromogenic substrate combinations, and serial removal of the antibody conjugates by low pH washes.

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页码：292 / 295

页数：4

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