Green synthesis of gold nanoparticles using aqueous extract of Mentha Longifolia leaf and investigation of its anti-human breast carcinoma properties in the in vitro condition

In this research, we reported an environment friendly approach for the synthesis of gold nanoparticles (Au NPs) using Mentha Longifolia leaf extract. The formation of the Au NPs was characterized by UV-visible and FT-IR spectroscopy, XRD, SEM, EDX and TEM analyses. The UV-Visible spectra of gold nanoparticles showed a surface plasmon resonance peak at 512 nm. The crystalline nature of gold nanoparticles was stablished by XRD diffraction pattern. TEM revealed the spherical shape with a mean particle size of 36.4 nm. Thereafter, biological performance of those biomolecule functionalized Au NPs was investigated. To survey the anti-human breast cancer effects of gold nanoparticles, MTT assay was used on the common breast cancer cell lines i.e., breast adenocarcinoma (MCF7), breast carcinoma (Hs 578Bst), breast infiltrating ductal cell carcinoma (Hs 319.T), and breast infiltrating lobular carcinoma (UACC-3133). The conversion was achieved in short reaction time with good to excellent yields in association with outstanding turnover frequency (TOF). In addition, the nanocomposite catalyst was easily recovered and recycled for 12 successive times without noticeable decrease in catalytic activity. Gold nanoparticles had high anti-breast cancer activities dose-dependently against MCF7, Hs 578Bst, Hs 319.T, and UACC-3133 cell lines. The best result of anti-breast cancer effects was seen in the case of the UACC-3133 cell line. It looks gold nanoparticles can be used for the treatment of several types of breast cancers in human. (C) 2020 Published by Elsevier B.V. on behalf of King Saud University.