Antibiotics for prophylaxis of Plasmodium falciparum infections:: In vitro activity of doxycycline against Senegalese isolates

被引:48
作者
Pradines, B
Spiegel, A
Rogier, C
Tall, A
Mosnier, J
Fusai, T
Trape, JF
Parzy, D
机构
[1] Serv Sante Armees, Inst Trop Med, Unite Parasitol, F-13998 Marseille Armees, France
[2] Inst Pasteur, Serv Epidemiol, Dakar, Senegal
[3] ORSTOM, Inst Rech Dev, Lab Paludol, Dakar, Senegal
关键词
D O I
10.4269/ajtmh.2000.62.82
中图分类号
R1 [预防医学、卫生学];
学科分类号
1004 ; 120402 ;
摘要
The in vitro activities of doxycycline, chloroquine, quinine, amodiaquine, artemether, pyrimethamine, and cycloguanil were evaluated against Plasmodium falciparum isolates from Senegal (Dielmo and Ndiop), using an isotopic, micro, drug susceptibility test. The 71 50% inhibitory concentration (IC,,) values for doxycycline ranged from 0.7 to 108.0 mu M and the geometric mean IC50 for the 71 isolates was 11.3 mu M (95% confidence interval = 9.5-13.4 mu M). The activity of doxycycline did not differ significantly (P = 0.0858) between the chloroquine-susceptible isolates and the chloroquine-resistant isolates. There was no in vitro correlation between the responses to doxycycline and those to artemether, chloroquine, quinine, amodiaquine, pyrimethamine, and cycloguanil, suggesting no in vitro cross-resistance among these drugs. Potency was increased by prolonged exposure. In 96-hr incubations. the activity of doxycycline was 4-5-fold more increased than in 48-hr incubations. The in vitro activity of doxycycline against intraerythrocytic stages of multidrug-resistant P. falciparum, its: action against the preerythrocytic forms, the lack of correlation between the responses in vitro of P. falciparum to doxycycline and the ether antimalarial drugs, and its original potential site of action are factors that favor its use as antimalarial drug.
引用
收藏
页码:82 / 85
页数:4
相关论文
共 34 条
[1]   Successful double-blinded, randomized, placebo-controlled field trial of azithromycin and doxycycline as prophylaxis for malaria in western Kenya [J].
Andersen, SL ;
Oloo, AJ ;
Gordon, DM ;
Ragama, OB ;
Aleman, GM ;
Berman, JD ;
Tang, DB ;
Dunne, MW ;
Shanks, GD .
CLINICAL INFECTIOUS DISEASES, 1998, 26 (01) :146-150
[2]   FAILURE OF CHLOROQUINE AND PROGUANIL PROPHYLAXIS IN TRAVELERS TO KENYA [J].
BARNES, AJ ;
ONG, ELC ;
DUNBAR, EM ;
MANDAL, BK ;
WILKINS, EGL .
LANCET, 1991, 338 (8778) :1338-1339
[3]   ACTIVITY IN-VITRO OF DOXYCYCLINE AGAINST MULTIDRUG-RESISTANT PLASMODIUM-FALCIPARUM [J].
BASCO, LK ;
LEBRAS, J .
TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE, 1993, 87 (04) :469-470
[4]  
BASCO LK, 1991, TROP MED PARASITOL, V42, P413
[5]  
BAUDON D, 1996, T R SOC TROP MED HYG, V93, P1
[6]  
CHILDS G E, 1987, Southeast Asian Journal of Tropical Medicine and Public Health, V18, P438
[7]   OXYGEN-DEPENDENT AND TIME-DEPENDENT EFFECTS OF ANTIBIOTICS AND SELECTED MITOCHONDRIAL INHIBITORS ON PLASMODIUM-FALCIPARUM IN CULTURE [J].
DIVO, AA ;
GEARY, TG ;
JENSEN, JB .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1985, 27 (01) :21-27
[8]   MEFLOQUINE FAILURE IN CHILD CONTRACTING FALCIPARUM-MALARIA IN WEST-AFRICA [J].
GAY, F ;
BINET, MH ;
BUSTOS, MDG ;
ROUVEIX, B ;
DANIS, M ;
ROY, C ;
GENTILINI, M .
LANCET, 1990, 335 (8681) :120-121
[9]  
GRAS C, 1993, B SOC PATHOL EXOT, V86, P52
[10]  
GRAS C, 1993, PRESSE MED, V22, P491