To see or not to see: Lateral organization of biological membranes and fluorescence microscopy

被引：293
作者：
Bagatolli, Luis A. [1 ]
机构：
[1] Univ So Denmark, Ctr Membrane Biophys, MEMPHYS, DK-5230 Odense M, Denmark
来源：
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES | 2006年 / 1758卷 / 10期
关键词：
LAURDAN; cholesterol; two photon excitation fluorescence microscopy; giant vesicle; lipid phase coexistence; raft;
D O I：
10.1016/j.bbamem.2006.05.019
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
In the last few years several experimental strategies based on epi-, confocal and two photon excitation fluorescence microscopy techniques have been employed to study the lateral structure of membranes using giant vesicles as model systems. This review article discusses the methodological aspects of the aforementioned experimental approaches, particularly stressing the information obtained by the use of UV excited fluorescent probes using two-photon excitation fluorescence microscopy. Additionally, the advantages of utilizing visual information, to correlate the lateral structure of compositionally simple membranes with complex situations, i.e., biological membranes, will be addressed. (c) 2006 Elsevier B.V. All rights reserved.
引用
收藏
页码：1541 / 1556
页数：16
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