Rapid method for resectioning of light microscopy sections for electron microscopy

被引：6
|
作者：
Kristof, Z
机构：
[1] Department of Plant Anatomy, Eötvös L. University, H-1088 Budapest
来源：
JOURNAL OF MICROSCOPY | 1997年 / 188卷
关键词：
embryo sac; re-embedding; resectioning; Torenia; transmission electron microscopy; ultramicrotomy;
D O I：
10.1046/j.1365-2818.1997.2280788.x
中图分类号：
TH742 [显微镜];
学科分类号：
摘要：
A rapid method is described for obtaining ultrathin sections from light microscopy sections, Five-micrometre epoxy sections, heat-flattened to slides, were affixed to the tips of plastic blocks by light-curable dental bond, and cured while still on the microscope stage by illumination with blue light for 2 min, Sections were detached from the slides by rapid cooling and then resectioned for electron microscopy.
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页码：88 / 91
页数：4
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