DDX4 (DEAD box polypeptide 4) colocalizes with cancer stem cell marker CD133 in ovarian cancers

被引：38

作者：

Kim, Ki Hyung ^{[1
,2
,3
]}

Kang, Yun-Jeong ^{[4
]}

Jo, Jin-Ok ^{[4
]}

Ock, Mee Sun ^{[4
]}

Moon, Soo Hyun ^{[1
,2
,3
]}

Suh, Dong Soo ^{[1
,2
,3
]}

Yoon, Man Soo ^{[1
,2
,3
]}

Park, Eun-Sil ^{[5
]}

Jeong, Namkung ^{[6
]}

Eo, Wan-Kyu ^{[7
]}

Kim, Heung Yeol ^{[8
]}

Cha, Hee-Jae ^{[4
,9
]}

机构：

[1] Pusan Natl Univ, Sch Med, Dept Obstet & Gynecol, Pusan, South Korea

[2] Pusan Natl Univ Hosp, Biomed Res Inst, Pusan, South Korea

[3] Pusan Natl Univ Hosp, Pusan Canc Ctr, Pusan, South Korea

[4] Kosin Univ, Coll Med, Dept Parasitol & Genet, Pusan 602702, South Korea

[5] Harvard Univ, Massachusetts Gen Hosp, Sch Med, Vincent Ctr Reprod Biol, Cambridge, MA 02138 USA

[6] Catholic Univ, Dept Obstet & Gynecol, Seoul, South Korea

[7] Kyung Hee Univ, Dept Internal Med, Seoul, South Korea

[8] Kosin Univ, Coll Med, Dept Obstet & Gynecol, Pusan 602702, South Korea

[9] Kosin Univ, Coll Med, Inst Med Sci, Pusan 602702, South Korea

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2014年 / 447卷 / 02期

基金：

新加坡国家研究基金会;

关键词：

DDX4; CD133; Ovarian cancer; Cancer stem cells; HEMATOPOIETIC STEM; GERM-CELLS; VASA; IDENTIFICATION; CD44; GENE; POPULATION; EXPRESSION; OOCYTE; AC133;

D O I：

10.1016/j.bbrc.2014.03.144

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

DDX4 (DEAD box polypeptide 4), characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), is an RNA helicase which is implicated in various cellular processes involving the alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. DDX4 is known to be a germ cell-specific protein and is used as a sorting marker of germline stem cells for the production of oocytes. A recent report about DDX4 in ovarian cancer showed that DDX4 is overexpressed in epithelial ovarian cancer and disrupts a DNA damage-induced G2 checkpoint. We investigated the relationship between DDX4 and ovarian cancer stem cells by analyzing the expression patterns of DDX4 and the cancer stem cell marker CD133 in ovarian cancers via tissue microarray. Both DDX4 and CD133 were significantly increased in ovarian cancer compared to benign tumors, and showed similar patterns of expression. In addition, DDX4 and CD133 were mostly colocalized in various types of ovarian cancer tissues. Furthermore, almost all CD133 positive ovarian cancer cells also express DDX4 whereas CD133-negative cells did not possess DDX4, suggesting a strong possibility that DDX4 plays an important role in cancer stem cells, and/or can be used as an ovarian cancer stem cell marker. (C) 2014 Elsevier Inc. All rights reserved.

引用

页码：315 / 322

页数：8

共 35 条

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