Use of solid-phase microextraction (SPME) for the determination of methadone and its main metabolite, EDDP, in plasma by gas chromatography-mass spectrometry

被引：30

作者：

Bermejo, AM ^{[1
]}

Seara, R

Lucas, ACD

Tabernero, MJ

Fernández, P

Marsili, R

机构：

[1] Fac Med, Forens Toxicol Serv, Dept Legal Med, Santiago De Compostela, Spain

[2] Univ Amazonas, Fac Pharm, Dept Clin Anal & Toxicol, Manaus, Amazonas, Brazil

[3] Catholic Univ Rome, Inst Legal Med, Rome, Italy

来源：

JOURNAL OF ANALYTICAL TOXICOLOGY | 2000年 / 24卷 / 01期

关键词：

D O I：

10.1093/jat/24.1.66

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A simple, rapid method for the determination of methadone and its metabolite 2-ethylene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP) in plasma using solid-phase microextraction (SPME) and gas chromatography-mass spectrometry is proposed. A 100-μm polydimethylsiloxane film fiber was exposed by immersion for 30 min in a diluted plasma solution (1:4 with buffer pH 9) containing both compounds and an internal standard (proadifen). Calibration curves were linear over the concentration range 50-2000 ng/mL. The analysis time was 45 min per sample. The determination of methadone and EDDP was subject to no interference. The performance of SPME was compared with that of liquid-liquid extraction, obtaining lower limits of detection for EDDP. The method using the two extraction procedures was applied to 10 plasma samples from methadone-treated patients.

引用

页码：66 / 69

页数：4

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