Field comparison of circulating antibody assays versus circulating antigen assays for the detection of schistosomiasis japonica in endemic areas of China

被引：32
作者：
Cai, Yu-Chun [1 ,2 ,3 ]
Xu, Jun-Fang [4 ]
Steinmann, Peter [5 ,6 ]
Chen, Shao-Hong [1 ,2 ,3 ]
Chu, Yan-Hong [1 ,2 ,3 ]
Tian, Li-Guang [1 ,2 ,3 ]
Chen, Mu-Xin [1 ,2 ,3 ]
Li, Hao [1 ,2 ,3 ]
Lu, Yan [1 ,2 ,3 ]
Zhang, Ling-Ling [1 ,2 ,3 ]
Zhou, Yang [1 ,2 ,3 ]
Chen, Jia-Xu [1 ,2 ,3 ]
机构：
[1] Chinese Ctr Dis Control & Prevent, Natl Inst Parasit Dis, Shanghai 200025, Peoples R China
[2] Minist Hlth, Key Lab Parasite & Vector Biol, Shanghai 200025, Peoples R China
[3] WHO Collaborating Ctr Malaria Schistosomiasis & F, Shanghai 200025, Peoples R China
[4] Hubei Univ Nationalities, Sch Med, Enshi 445000, Hubei, Peoples R China
[5] Swiss Trop & Publ Hlth Inst, Dept Epidemiol & Publ Hlth, CH-4051 Basel, Switzerland
[6] Univ Basel, CH-4051 Basel, Switzerland
来源：
PARASITES & VECTORS | 2014年 / 7卷
关键词：
Schistosoma japonicum; Circulating antibody; Circulating antigen; China; REPUBLIC-OF-CHINA; REAGENT STRIP TEST; SUB-SAHARAN AFRICA; CATHODIC ANTIGEN; MANSONI INFECTION; EGG COUNTS; ANODIC ANTIGEN; DIAGNOSIS; IMMUNODIAGNOSIS; TRANSMISSION;
D O I：
10.1186/1756-3305-7-138
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
Background: Schistosomiasis remains a serious public health problem in affected countries, and routine, highly sensitive and cost-effective diagnostic methods are lacking. We evaluated two immunodiagnostic techniques for the detection of Schistosoma japonicum infections: circulating antibody and circulating antigen assays. Methods: A total of 1864 individuals (between 6 and 72 years old) residing in five administrative villages in Hubei province were screened by serum examination with an indirect hemagglutination assay (IHA). The positive individuals (titer >= 20 in IHA) were reconfirmed by stool examination with the Kato-Katz method (three slides from a single stool specimen). Samples of good serum quality and a volume above 0.5 ml were selected for further testing with two immunodiagnostic antibody (DDIA and ELISA) and two antigen (ELISA) assays. Results: The average antibody positive rate in the five villages was 12.7%, while the average parasitological prevalence was 1.50%; 25 of the 28 egg-positive samples were also circulating antigen-positive. Significant differences were observed between the prevalence according to the Kato-Katz method and all three immunodiagnostic antibody assays (P-value <0.0001). Similar differences were observed between the Kato-Katz method and the two immunodiagnostic antigen assays (P-value <0.0001) and between the antigen and antibody assays (P-value <0.0001). Conclusion: Both circulating antibody and circulating antigen assays had acceptable performance characteristics. Immunodiagnostic techniques to detect circulating antigens have potential to be deployed for schistosomiasis japonica screening in the endemic areas.
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