Immune Signature of Enhanced Functional Avidity CD8+ T Cells in vivo Induced by Vaccinia Vectored Vaccine

被引:9
作者
Hu, Zhidong [1 ]
Zhu, Lingyan [1 ]
Wang, Jing [1 ]
Wan, Yanmin [1 ]
Yuan, Songhua [1 ]
Chen, Jian [1 ]
Ding, Xiangqing [1 ]
Qiu, Chenli [1 ]
Zhang, Xiaoyan [1 ,2 ,3 ]
Qiu, Chao [2 ,3 ]
Xu, Jianqing [1 ,2 ,3 ]
机构
[1] Fudan Univ, Shanghai Publ Hlth Clin Ctr, Shanghai, Peoples R China
[2] Fudan Univ, Inst Biomed Sci, Shanghai, Peoples R China
[3] Fudan Univ, Key Lab Med Mol Virol MOE MOH, Shanghai, Peoples R China
基金
中国国家自然科学基金;
关键词
TOLL-LIKE RECEPTOR; ANTIGEN SENSITIVITY; POLYFUNCTIONALITY; PROGRESSION; EXPRESSION; EPITOPES; PROTEIN;
D O I
10.1038/srep41558
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Functional avidity of T cells is a critical determinant for clearing viral infection and eliminating tumor. Understanding how functional avidity is maintained in T cells is imperative for immunotherapy. However, studies systematically characterize T cell with high functional avidity induced in vivo are still lacking. Previously, we and others found vaccinia vectored vaccine (VACV) induced antigen-specific CD8(+) T cells with relatively high functional avidity to those from DNA vaccine. Herein, we used functional, immune phenotyping and transcriptomic studies to define the immune signature of these CD8(+) T cells with high functional avidity. Antigen-specific CD8(+) T cells induced by VACV executed superior in vivo killing activity and displayed a distinct transcriptional profile, whereas no significantly differences were found in composition of memory sub-populations and cytokine poly-functionality. Transcriptional analyses revealed unique features of VACV induced CD8(+) T cells in several biological processes, including transport, cell cycle, cell communication and metabolic processes. In summary, we characterize CD8(+) T cells of high functional avidity induced in vivo by VACV, which not only improves our understanding of adaptive T cell immunity in VACV vaccination, but also provides clues to modulate functional avidity of CD8(+) T cells for T cell based immunotherapy.
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页数:12
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