The effect of sterilization methods on electronspun poly(lactide-co-glycolide) and subsequent adhesion efficiency of mesenchymal stem cells

被引：33

作者：

Braghirolli, Daikelly Iglesias ^{[1
,2
]}

Steffens, Daniela ^{[1
,2
]}

Quintiliano, Kerlin ^{[1
,3
]}

Xavier Acasigua, Gerson Arisoly ^{[1
,4
]}

Gamba, Douglas ^{[5
]}

Fleck, Roland A. ^{[6
]}

Petzhold, Cesar L. ^{[5
]}

Pranke, Patricia ^{[1
,2
,7
]}

机构：

[1] Univ Fed Rio Grande do Sul, Fac Pharm, Hematol & Stem Cell Lab, BR-90610000 Porto Alegre, RS, Brazil

[2] Univ Fed Rio Grande do Sul, Post Grad Programme Mat Sci, BR-91501970 Porto Alegre, RS, Brazil

[3] Univ Fed Rio Grande do Sul, Post Grad Programme Neurosci, BR-90050170 Porto Alegre, RS, Brazil

[4] Univ Fed Rio Grande do Sul, Post Grad Programme Dent, BR-90035003 Porto Alegre, RS, Brazil

[5] Univ Fed Rio Grande do Sul, Inst Chem, BR-91501970 Porto Alegre, RS, Brazil

[6] Natl Inst Biol Stand & Controls, Potters Bar EN6 3QG, Herts, England

[7] Stem Cell Res Inst, BR-90020010 Porto Alegre, RS, Brazil

来源：

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS | 2014年 / 102卷 / 04期

关键词：

PLGA; biomaterials; electrospinning; nanofibers; sterilization; IRRADIATION; SCAFFOLDS;

D O I：

10.1002/jbm.b.33049

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

The sterilization of scaffolds is an essential step for tissue engineering in vitro and, mainly, clinical biomaterial use. However, this process can cause changes in the structure and surface of the scaffolds. Therefore, the objective of this study was to investigate the effect of sterilization by ethanol, ultraviolet radiation (UVR) or antimicrobial solution (AMS) on poly(lactide-co-glycolide) (PLGA) scaffolds produced by the electrospinning technique. The properties of nanofibers and the cellular adhesion of mesenchymal stem cells to the scaffolds were analyzed after the treatments. All methods generated sterile scaffolds but showed some kind of damage to the scaffolds. Ethanol and AMS caused changes in the morphology and scaffold dimensions, which were not observed when using the UVR method. However, UVR caused a greater reduction in polymeric molecular weight, which increased proportionally with exposure time of treatment. Nanofibers sterilized with AMS for 1 h and 2 h showed greater cellular adhesion than the other methods, demonstrating their potential as a method for sterilizing PLGA nanofibers. (c) 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 102B: 700-708, 2014.

引用

页码：700 / 708

页数：9

共 22 条

[1] Effects of sterilisation method on surface topography and in-vitro cell behaviour of electrostatically spun scaffolds [J].

Andrews, Kirstie D. ;

Hunt, John A. ;

Black, Richard A. .

BIOMATERIALS, 2007, 28 (06) :1014-1026

[2] Sterilization, toxicity, biocompatibility and clinical applications of polylactic acid polyglycolic acid copolymers [J].

Athanasiou, KA ;

Niederauer, GG ;

Agrawal, CM .

BIOMATERIALS, 1996, 17 (02) :93-102

[3] The Isolation of Stem Cells from Human Deciduous Teeth Pulp Is Related to the Physiological Process of Resorption [J].

Bernardi, Lisiane ;

Luisi, Simone Bonato ;

Fernandes, Raquel ;

Dalberto, Tiago Pires ;

Valentim, Lauren ;

Bogo Chies, Jose Artur ;

Medeiros Fossati, Anna Christina ;

Pranke, Patricia .

JOURNAL OF ENDODONTICS, 2011, 37 (07) :973-979

[4]

Costa L.M. M., 2012, J. Biomater. Nanobiotech, V3, P92, DOI [10.1016/j.addr.2009.09.002, DOI 10.4236/JBNB.2012.31012, 10.4236/jbnb.2012.31012]

[5] Effect of sterilisation by gamma irradiation on the ability of polycaprolactone (PCL) to act as a scaffold material [J].

Cottam, Emily ;

Hukins, David W. L. ;

Lee, Kenneth ;

Hewitt, Christopher ;

Jenkins, Mike J. .

MEDICAL ENGINEERING & PHYSICS, 2009, 31 (02) :221-226

[6] Degradation of electrospun nanofiber scaffold by short wave length ultraviolet radiation treatment and its potential applications in tissue engineering [J].

Dong Yixiang ;

Yong, Thomas ;

Liao, Susan ;

Chan, Casey K. ;

Ramakrishna, S. .

TISSUE ENGINEERING PART A, 2008, 14 (08) :1321-1329

[7] Nanofibers Offer Alternative Ways to the Treatment of Skin Infections [J].

Heunis, T. D. J. ;

Dicks, L. M. T. .

JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY, 2010,

[8] Optimizing the sterilization of PLGA scaffolds for use in tissue engineering [J].

Holy, CE ;

Cheng, C ;

Davies, JE ;

Shoichet, MS .

BIOMATERIALS, 2001, 22 (01) :25-31

[9] Controlled cellular orientation on PLGA microfibers with defined diameters [J].

Hwang, C. M. ;

Park, Y. ;

Park, J. Y. ;

Lee, K. ;

Sun, K. ;

Khademhosseini, A. ;

Lee, S. H. .

BIOMEDICAL MICRODEVICES, 2009, 11 (04) :739-746

[10] Periodontal ligament cellular structures engineered with electrospun poly(DL-lactide-co-glycolide) nanofibrous membrane scaffolds [J].

Inanc, Buelend ;

Arslan, Y. Emre ;

Seker, Suekran ;

Elcin, A. Eser ;

Elcin, Y. Murat .

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 2009, 90A (01) :186-195

← 1 2 3 →