Isolation and characterization of acid- and pepsin-soluble collagens from Lagocephalus gloveri skin

Fish skin is a major by-product of the fish processing industry, causing wastage and pollution, and could provide a valuable source of collagen which has wide biomedical and pharmaceutical applications. Acid soluble collagen (ASC) and pepsin soluble collagen (PSC) from the skin of Lagocephalus gloveri showed the yield of 8 and 17% respectively and were characterized to type I collagen, containing two different a chains (alpha 1 and alpha 2) and one beta chain. Fourier transform infrared (FTIR) spectra of ASC and PSC were observed and were found to be similar. The denaturation temperature (T-d) of ASC and PSC was found to be 29.3 degrees C and 28.4 degrees C respectively. There was no major difference in solubility observed for both ASC and PSC at various pH and for different NaCl concentrations. Hence, there is possibility to use collagen from Lagocephalus gloveri skin as an alternative source of collagen for use in various applications.