Dihydromyricetin induces cell apoptosis via a p53-related pathway in AGS human gastric cancer cells

被引：32

作者：

Ji, F. J. ^{[1
]}

Tian, X. F. ^{[1
]}

Liu, X. W. ^{[2
]}

Fu, L. B. ^{[3
]}

Wu, Y. Y. ^{[1
]}

Fang, X. D. ^{[1
]}

Jin, H. Y. ^{[1
]}

机构：

[1] Jilin Univ, China Japan Friendship Hosp, Dept Gen Surg, Changchun 130023, Peoples R China

[2] Jilin Cent Hosp, Dept Gen Surg, Changchun, Jilin, Peoples R China

[3] Peoples Hosp Panan Cty, Dept Gen Surg, Hangzhou, Zhejiang, Peoples R China

来源：

GENETICS AND MOLECULAR RESEARCH | 2015年 / 14卷 / 04期

关键词：

Dihydromyricetin; Gastric cancer; Cytotoxicity; Apoptosis; p53; DEATH; LIFE; MITOCHONDRIA; PROTEINS;

D O I：

10.4238/2015.December.1.7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The aim of the present study was to determine the anti-proliferative and pro-apoptotic effects of dihydromyricetin (DHM) on the AGS human gastric cancer cells and their underlying mechanisms. The effects of DHM on AGS cells were evaluated by using 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), lactate dehydrogenase, and Annexin V/propidium iodide (PI) double-staining assays. The underlying mechanisms were determined by using quantitative real-time polymerase chain reaction. The results demonstrated that DHM significantly (P < 0.05) inhibited AGS cell proliferation and induced cell cytotoxicity in a dose-and time-dependent manner. Additionally, Annexin V/PI double-staining assay showed that DHM promoted cell apoptosis in both, early and late stages. Furthermore, DHM also regulated the expression of apoptotic genes such as p53 and B-cell lymphoma-2 (bcl-2) in a dose- and time-dependent manner. In conclusion, this is the first report demonstrating the anticancer and pro-apoptosis effects of DHM on AGS human gastric cancer cells. The results strongly suggest that DHM may be a potential therapeutic candidate for the treatment of gastric cancer.

引用

页码：15564 / 15571

页数：8

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