Comparative analysis of the blood transcriptomes between wolves and dogs

被引：22

作者：

Yang, X. ^{[1
]}

Zhang, H. ^{[1
]}

Shang, J. ^{[2
]}

Liu, G. ^{[1
]}

Xia, T. ^{[1
]}

Zhao, C. ^{[1
]}

Sun, G. ^{[1
]}

Dou, H. ^{[3
]}

机构：

[1] Qufu Normal Univ, Coll Life Sci, Jingxuan West Rd 57, Qufu 273165, Shandong, Peoples R China

[2] Qufu Normal Univ, Coll Informat Sci & Engn, Yantai North Rd 80, Rizhao 276826, Shandong, Peoples R China

[3] Dailake Natl Nat Reserve, Manzhouli Rd 16, Hulunbuir 021000, Inner Mongolia, Peoples R China

来源：

ANIMAL GENETICS | 2018年 / 49卷 / 04期

关键词：

Canis lupus; Canis lupus familiaris; differentially expressed gene; RNA-Seq; transcriptome; RNA-SEQ; EXPRESSION; GENE; SELECTION;

D O I：

10.1111/age.12675

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

Dogs were domesticated by human and originated from wolves. Their evolutionary relationships have attracted much scientific interest due to their genetic affinity but different habitats. To identify the differences between dogs and wolves associated with domestication, we analysed the blood transcriptomes of wolves and dogs by RNA-Seq. We obtained a total of 30.87Gb of raw reads from two dogs and three wolves using RNA-Seq technology. Comparisons of the wolf and dog transcriptomes revealed 524 genes differentially expressed genes between them. We found that some genes related to immune function (DCK, ICAM4, GAPDH and BSG) and aerobic capacity (HBA1, HBA2 and HBB) were more highly expressed in the wolf. Six differentially expressed genes related to the innate immune response (CCL23, TRIM10, DUSP10, RAB27A, CLEC5A and GCH1) were found in the wolf by a Gene Ontology enrichment analysis. Immune system development was also enriched only in the wolf group. The ALAS2, HMBS and FECH genes, shown to be enriched by the Kyoto Encyclopedia of Genes and Genomes analysis, were associated with the higher aerobic capacity and hypoxia endurance of the wolf. The results suggest that the wolf might have greater resistance to pathogens, hypoxia endurance and aerobic capacity than dogs do.

引用

页码：291 / 302

页数：12

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