Carbon Nanotubes Activate Limulus Amebocyte Lysate Coagulation by Interface Adsorption

被引：9

作者：

Yang, Man ^{[1
]}

Nie, Xin ^{[2
,3
]}

Meng, Jie ^{[2
,3
]}

Liu, Jian ^{[2
,3
]}

Sun, Zhiwei ^{[1
]}

Xu, Haiyan ^{[2
,3
]}

机构：

[1] Capital Med Univ, Sch Publ Hlth, Beijing 100069, Peoples R China

[2] Chinese Acad Med Sci, Inst Basic Med Sci, Beijing 100005, Peoples R China

[3] Peking Union Med Coll, Sch Basic Med, Beijing 100005, Peoples R China

来源：

ACS APPLIED MATERIALS & INTERFACES | 2017年 / 9卷 / 10期

基金：

北京市自然科学基金; 中央高校基本科研业务费专项资金资助;

关键词：

carbon nanotubes; endotoxin contamination; limulus amebocyte lysate; interface interaction; protein adsorption; enzyme activation; FUNCTIONAL EXCIPIENTS; NANOPARTICLES; ENDOTOXIN; CONTAMINATION; CHALLENGES; DELIVERY; ENHANCE;

D O I：

10.1021/acsami.7b00543

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

Limulus amebocyte lysate (LAL) assay is worldwide requested in the assessment of endotoxin contamination for biomaterials. As carbon nanotubes are one major nanomaterial with multiple potentials in biomedical application, here we investigate whether oxidized multiwalled carbon nanotubes MWCNT) interferes the assessment by LAL assays. We showed that the endotoxin free O-MWCNT dispersing in aqueous solutions could activate both the gel-clotting and the end-point chromogenic LAL assay by converting coagulogen into coagulin through interfacial interactions between O-MWCNT and enzymes in the assays. In conclusion, the O-MWCNT could induce false positive results by activating the enzyme cascade of LAL.

引用

页码：8450 / 8454

页数：5

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