A Periosteum-Inspired 3D Hydrogel-Bioceramic Composite for Enhanced Bone Regeneration

被引：25

作者：

Chun, Yong Yao ^{[1
]}

Wang, Jun Kit ^{[4
,5
]}

Tan, Nguan Soon ^{[3
,6
,7
]}

Chan, Peggy Puk Yik ^{[8
,9
]}

Tan, Timothy Thatt Yang ^{[1
]}

Choong, Cleo ^{[2
,3
]}

机构：

[1] Nanyang Technol Univ, Sch Chem & Biomed Engn, 62 Nanyang Dr, Singapore 637459, Singapore

[2] Nanyang Technol Univ, Sch Mat Sci & Engn, 50 Nanyang Ave, Singapore 639798, Singapore

[3] KK Womens & Children Hosp, KK Res Ctr, 100 Bukit Timah Rd, Singapore 229899, Singapore

[4] Nanyang Technol Univ, Residues & Resource Reclamat Ctr, Nanyang Environm & Water Res Inst, 1 Cleantech Loop, Singapore 637141, Singapore

[5] Nanyang Technol Univ, Interdisciplinary Grad Sch, 50 Nanyang Ave, Singapore 639798, Singapore

[6] Nanyang Technol Univ, Sch Biol Sci, 60 Nanyang Dr, Singapore 637551, Singapore

[7] ASTAR, Inst Mol & Cell Biol, Proteos, 61 Biopolis Dr, Singapore 138673, Singapore

[8] Swinburne Univ Technol, Dept Telecommun Elect Robot & Biomed Engn, Hawthorn, Vic 3122, Australia

[9] Melbourne Ctr Nanofabricat, Clayton, Vic 3168, Australia

来源：

MACROMOLECULAR BIOSCIENCE | 2016年 / 16卷 / 02期

基金：

新加坡国家研究基金会;

关键词：

biocompatibility; biomaterial; biomineralization; biopolymers; hydrogels; GRAFT; HYDROXYAPATITE; EXTRACTION; POROSITY;

D O I：

10.1002/mabi.201500258

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A 3D injectable hydrogel-bioceramic composite consisting of gelatin-3-(4-hydroxyphenyl) propionic acid (Gtn-HPA) and carboxymethyl cellulose-tyramine (CMC-Tyr), incorporated with fish scale-derived calcium phosphate (CaP), is developed for bone applications. The hydrogel-bioceramic composite has significantly improved the elastic modulus compared to the non-filled hydrogel, of which the addition of 10w/v% CaP showed zero order fluorescein isothiocyanate (FITC)-dextran release profile and a significantly higher proliferation rate of encapsulated cells. All the samples promote the nucleation and growth of CaP minerals when exposed to 1x SBF. Overall, the hydrogel-bioceramic composite with 10w/v% CaP can potentially be used as a periosteum-mimicking membrane to facilitate bone regeneration.

引用

页码：276 / 287

页数：12

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