Peptide and protein sequence analysis by electron transfer dissociation mass spectrometry

被引：1870
作者：
Syka, JEP
Coon, JJ
Schroeder, MJ
Shabanowitz, J
Hunt, DF
机构：
[1] Univ Virginia, Dept Chem, Charlottesville, VA 22901 USA
[2] Univ Virginia, Engn Phys Program, Charlottesville, VA 22901 USA
[3] Thermo Electron, San Jose, CA 95134 USA
[4] Univ Virginia, Hlth Sci Ctr, Dept Pathol, Charlottesville, VA 22908 USA
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2004年 / 101卷 / 26期
关键词：
D O I：
10.1073/pnas.0402700101
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Peptide sequence analysis using a combination of gas-phase ion/ion chemistry and tandem mass spectrometry (MS/MS) is demonstrated. Singly charged anthracene anions transfer an electron to multiply protonated peptides in a radio frequency quadrupole linear ion trap (QLT) and induce fragmentation of the peptide backbone along pathways that are analogous to those observed in electron capture dissociation. Modifications to the QLT that enable this ion/ion chemistry are presented, and automated acquisition of high-quality, single-scan electron transfer dissociation MS/MS spectra of phosphopeptides separated by nanoflow HPLC is described.
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收藏
页码：9528 / 9533
页数：6
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