Spectral Domain Optical Coherence Tomography: An In Vivo Imaging Protocol for Assessing Retinal Morphology in Adult Zebrafish

被引：10

作者：

Toms, Maria ^{[1
]}

Tracey-White, Dhani ^{[1
]}

Muhundhakumar, Dhakshi ^{[1
]}

Sprogyte, Lina ^{[1
]}

Dubis, Adam M. ^{[1
,2
]}

Moosajee, Mariya ^{[1
,2
]}

机构：

[1] UCL Inst Ophthalmol, Dept Ocular Biol & Therapeut, 11-43 Bath St, London EC1V 9EL, England

[2] Moorfields Eye Hosp NHS Fdn Trust, London, England

来源：

ZEBRAFISH | 2017年 / 14卷 / 02期

基金：

英国惠康基金;

关键词：

retina; spectral domain optical coherence tomography; cone mosaic; adult zebrafish; VISUAL-SYSTEM; PHOTORECEPTORS; IDENTIFICATION; DEGENERATION; REGENERATION; MODELS; GENOME; CELLS;

D O I：

10.1089/zeb.2016.1376

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The present study outlines a protocol for examining retinal structure in zebrafish, a popular model organism for ocular studies, using spectral domain optical coherence tomography (SD-OCT). We demonstrate how this live imaging modality can be used to obtain high quality images of several retinal features, including the optic nerve, retinal vasculature, and the cone photoreceptor mosaic. Retinal histology sections were obtained from imaged fish for comparison with SD-OCT cross-sectional B-scans. Voronoi domain analysis was used to assess cone photoreceptor packing regularity at 3, 6, and 12 months. SD-OCT is an effective in vivo technique for studying the adult zebrafish retina and can be applied to disease models for longitudinal serial monitoring.

引用

页码：118 / 125

页数：8

共 39 条

[1] Optical coherence tomography - current and future applications
Adhi, Mehreen
Duker, Jay S.
[J]. CURRENT OPINION IN OPHTHALMOLOGY, 2013, 24 (03) : 213 - 221
[2] Ontogeny of Cone Photoreceptor Mosaics in Zebrafish
Allison, W. Ted
Barthel, Linda K.
Skebo, Kristina M.
Takechi, Masaki
Kawamura, Shoji
Raymond, Pamela A.
[J]. JOURNAL OF COMPARATIVE NEUROLOGY, 2010, 518 (20) : 4182 - 4195
[3] Spectral-Domain Optical Coherence Tomography as a Noninvasive Method to Assess Damaged and Regenerating Adult Zebrafish Retinas
Bailey, Travis J.
Davis, Darin H.
Vance, Joseph E.
Hyde, David R.
[J]. INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2012, 53 (06) : 3126 - 3138
[4] Adaptive optics retinal imaging reveals S-cone dystrophy in tritan color-vision deficiency
Baraas, Rigmor C.
Carroll, Joseph
Gunther, Karen L.
Chung, Mina
Williams, David R.
Foster, David H.
Neitz, Maureen
[J]. JOURNAL OF THE OPTICAL SOCIETY OF AMERICA A-OPTICS IMAGE SCIENCE AND VISION, 2007, 24 (05) : 1438 - 1447
[5] The adult zebrafish retina: In vivo optical sectioning with Confocal Scanning Laser Ophthalmoscopy and Spectral-Domain Optical Coherence Tomography
Bell, Brent A.
Yuan, Alex
Dicicco, Rose M.
Fogerty, Joseph
Lessieur, Emma M.
Perkins, Brian D.
[J]. EXPERIMENTAL EYE RESEARCH, 2016, 153 : 65 - 78
[6] THE DEVELOPMENT OF PHOTORECEPTORS IN THE ZEBRAFISH, BRACHYDANIO-RERIO .1. STRUCTURE
BRANCHEK, T
BREMILLER, R
[J]. JOURNAL OF COMPARATIVE NEUROLOGY, 1984, 224 (01) : 107 - 115
[7] Zebrafish-on the move towards ophthalmological research
Chhetri, J.
Jacobson, G.
Gueven, N.
[J]. EYE, 2014, 28 (04) : 367 - 380
[8] Rapid, Accurate, and Non-Invasive Measurement of Zebrafish Axial Length and Other Eye Dimensions Using SD-OCT Allows Longitudinal Analysis of Myopia and Emmetropization
Collery, Ross F.
Veth, Kerry N.
Dubis, Adam M.
Carroll, Joseph
Link, Brian A.
[J]. PLOS ONE, 2014, 9 (10):
[9] Effects of Intraframe Distortion on Measures of Cone Mosaic Geometry from Adaptive Optics Scanning Light Ophthalmoscopy
Cooper, Robert F.
Sulai, Yusufu N.
Dubis, Adam M.
Chui, Toco Y.
Rosen, Richard B.
Michaelides, Michel
Dubra, Alfredo
Carroll, Joseph
[J]. TRANSLATIONAL VISION SCIENCE & TECHNOLOGY, 2016, 5 (01):
[10] Retinal Regeneration Following OCT-Guided Laser Injury in Zebrafish
DiCicco, Rose M.
Bell, Brent A.
Kaul, Charles
Hollyfield, Joe G.
Anand-Apte, Bela
Perkins, Brian D.
Tao, Yuankai K.
Yuan, Alex
[J]. INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2014, 55 (10) : 6281 - 6288

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