A new role for RU486 (mifepristone): it protects sperm from premature capacitation during cryopreservation in buffalo

被引：30

作者：

Dalal, Jasmer ^{[1
,2
]}

Kumar, Pradeep ^{[1
]}

Chandolia, R. K. ^{[2
]}

Pawaria, Shikha ^{[1
]}

Rajendran, Rasika ^{[1
]}

Sheoran, Suman ^{[1
]}

Andonissamy, Jerome ^{[1
]}

Kumar, Dharmendra ^{[1
]}

机构：

[1] Cent Inst Res Buffaloes, ICAR, Anim Physiol & Reprod Div, Hisar 125001, Haryana, India

[2] Lala Lajpat Rai Univ Vet & Anim Sci, Dept Vet Gynaecol & Obstet, Hisar 125001, Haryana, India

来源：

SCIENTIFIC REPORTS | 2019年 / 9卷 / 1期

关键词：

IN-VITRO; MOUSE SPERMATOZOA; ACROSOME REACTION; WATER-BUFFALO; BINDING ASSAY; PROGESTERONE; SEMEN; CRYOCAPACITATION; ANTIOXIDANT; CHOLESTEROL;

D O I：

10.1038/s41598-019-43038-4

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The objective of this study was to determine the mechanism by which RU 486 (mifepristone) protects sperm to undergo premature capacitation during cryopreservation. For this, semen ejaculate (n = 20) was divided into four equal fractions and diluted using egg yolk-based extender supplemented with different concentrations of RU 486 (0, 5, 10 and 20 mu M) and cryopreserved. We found that RU 486 did not impair the post-thaw sperm kinetics and motility but prevented cholesterol efflux, calcium influx, and protected CatSper channels during cryopreservation. The RU 486 protected sperm from premature capacitation which was confirmed by intracellular calcium level, expression of tyrosine phosphorylated proteins (75 and 80 kDa) and CTC (chlortetracycline) assay. Furthermore, antioxidant ability of RU 486 was reflected by the ferric reducing ability, lower production of sperm malondialdehyde and intracellular reactive oxygen species. Also, we demonstrated that RU 486 treated sperm underwent normal capacitation, zona pellucida binding and zygote cleavage indicating normal fertilizing ability of sperm. In conclusion, we report a new role of RU 486 in protecting buffalo sperm from premature capacitation during cryopreservation.

引用

页数：10

共 48 条

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