Biodegradation of kraft lignin by a newly isolated anaerobic bacterial strain, Acetoanaerobium sp WJDL-Y2

被引：61

作者：

Duan, J. ^{[1
]}

Huo, X. ^{[1
]}

Du, W. J. ^{[1
]}

Liang, J. D. ^{[1
,2
]}

Wang, D. Q. ^{[3
]}

Yang, S. C. ^{[1
]}

机构：

[1] Xi An Jiao Tong Univ, Dept Environm Engn, Xian 710049, Peoples R China

[2] Chinese Acad Sci, Cold & Arid Reg Environm & Engn Res Inst, State Key Lab Frozen Soil Engn, Lanzhou, Peoples R China

[3] Xian Univ Technol, State Key Lab Base Ecohydraul Engn Arid Area, Xian, Peoples R China

来源：

LETTERS IN APPLIED MICROBIOLOGY | 2016年 / 62卷 / 01期

关键词：

16S rRNA gene sequencing; Acetoanaerobium sp WJDL-Y2; GC-MS; growth kinetics; kraft lignin degradation; response surface methodology; CHROMATOGRAPHY-MASS-SPECTROMETRY; PAPER-MILL EFFLUENT; PHENOL BIODEGRADATION; LIMITED DEGRADATION; WASTE-WATER; PULP; OPTIMIZATION; DECOLORIZATION; FERMENTATION; INDUSTRIAL;

D O I：

10.1111/lam.12508

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

An anaerobic kraft lignin (KL)-degrading bacterial strain was isolated from sludge of a pulp and paper mill. It was characterized as Acetoanaerobium sp. WJDL-Y2 by 16S rRNA gene sequencing. The maximum KL degradation capability of strain Y2 was determined to be 24.9% on a COD basis under an optimal condition with temperature of 31.5 degrees C, initial pH of 6.8 and KL to nitrogen (as NH4Cl) ratio of 6.5 by mass. Growth kinetic studies showed that the KL tolerance of strain Y2 was relatively high (K-i = 8120.45 mg l(-1)). Analysing KL degradation products by GC-MS revealed the formation of low-molecular-weight aromatic compounds (LMWACs), including benzenepropanoic acid, syringic acid and ferulic acid. This indicates that strain Y2 can oxidize lignin structure's p-hydroxyphenyl (H) units, guaiacyl (G) units and syringyl (S). In addition, the inoculated sample also contained low-molecular acid compounds, such as hexanoic acid, adipic acid and 2-hydroxybutyric acid, further validating strain Y2's ability to degrade KL.

引用

页码：55 / 62

页数：8

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