Performance of Panfungal-and Specific-PCR-Based Procedures for Etiological Diagnosis of Invasive Fungal Diseases on Tissue Biopsy Specimens with Proven Infection: a 7-Year Retrospective Analysis from a Reference Laboratory

被引：45
作者：
Buitrago, M. J. [1 ]
Bernal-Martinez, L. [1 ]
Castelli, M. V. [1 ]
Rodriguez-Tudela, J. L. [1 ]
Cuenca-Estrella, M. [1 ]
机构：
[1] Inst Salud Carlos III, Natl Ctr Microbiol, Dept Mycol, Madrid, Spain
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 2014年 / 52卷 / 05期
关键词：
REAL-TIME PCR; ASSAY; DETECT; DNA;
D O I：
10.1128/JCM.00328-14
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
A retrospective analysis of real-time PCR (RT-PCR) results for 151 biopsy samples obtained from 132 patients with proven invasive fungal diseases was performed. PCR-based techniques proved to be fast and sensitive and enabled definitive diagnosis in all cases studied, with detection of a total of 28 fungal species.
引用
收藏
页码：1737 / 1740
页数：4
相关论文
共 15 条
[1]   Development of a single tube multiplex real-time PCR to detect the most clinically relevant Mucormycetes species [J].
Bernal-Martinez, L. ;
Buitrago, M. J. ;
Castelli, M. V. ;
Rodriguez-Tudela, J. L. ;
Cuenca-Estrella, M. .
CLINICAL MICROBIOLOGY AND INFECTION, 2013, 19 (01) :E1-E7
[2]   Detection of invasive infection caused by Fusarium solani and non-Fusarium solani species using a duplex quantitative PCR-based assay in a murine model of fusariosis [J].
Bernal-Martinez, Leticia ;
Buitrago, Maria J. ;
Castelli, Maria V. ;
Rodriguez-Tudela, Juan L. ;
Cuenca-Estrella, Manuel .
MEDICAL MYCOLOGY, 2012, 50 (03) :270-275
[3]   Detection of imported histoplasmosis in serum of HIV-infected patients using a real-time PCR-based assay [J].
Buitrago, M. J. ;
Berenguer, J. ;
Mellado, E. ;
Rodriguez-Tudela, J. L. ;
Cuenca-Estrella, M. .
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 2006, 25 (10) :665-668
[4]   Efficacy of DNA amplification in tissue biopsy samples to improve the detection of invasive fungal disease [J].
Buitrago, M. J. ;
Aguado, J. M. ;
Ballen, A. ;
Bernal-Martinez, L. ;
Prieto, M. ;
Garcia-Reyne, A. ;
Garcia-Rodriguez, J. ;
Rodriguez-Tudela, J. L. ;
Cuenca-Estrella, M. .
CLINICAL MICROBIOLOGY AND INFECTION, 2013, 19 (06) :E271-E277
[5]   Utility of Real-time PCR for the detection of Paracoccidioides brasiliensis DNA in the diagnosis of imported paracoccidioidomycosis [J].
Buitrago, M. J. ;
Merino, P. ;
Puente, S. ;
Gomez-Lopez, A. ;
Arribi, A. ;
Zancope-Oliveira, R. M. ;
Gutierrez, M. C. ;
Rodriguez-Tudela, J. L. ;
Cuenca-Estrella, M. .
MEDICAL MYCOLOGY, 2009, 47 (08) :879-882
[6]   Development and validation of a quantitative PCR assay for diagnosis of scedosporiosis [J].
Castelli, Maria V. ;
Buitrago, Maria J. ;
Bernal-Martinez, Leticia ;
Gomez-Lopez, Alicia ;
Rodriguez-Tudela, Juan L. ;
Cuenca-Estrella, Manuel .
JOURNAL OF CLINICAL MICROBIOLOGY, 2008, 46 (10) :3412-3416
[7]   Update on the epidemiology and diagnosis of invasive fungal infection [J].
Cuenca-Estrella, Manuel ;
Bernal-Martinez, Leticia ;
Jose Buitrago, Maria ;
Victoria Castelli, Maria ;
Gomez-Lopez, Alicia ;
Zaragoza, Oscar ;
Rodriguez-Tudela, Juan Luis .
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS, 2008, 32 :S143-S147
[8]   Detection and investigation of invasive mould disease [J].
Cuenca-Estrella, Manuel ;
Bassetti, Matteo ;
Lass-Floerl, Cornelia ;
Racil, Zdenek ;
Richardson, Malcolm ;
Rogers, Thomas R. .
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2011, 66 :i15-i24
[9]   Endocarditis Caused by Arthrographis kalrae [J].
de Diego Candela, Javier ;
Forteza, Alberto ;
Garcia, Diana ;
Prieto, Gustavo ;
Bellot, Raquel ;
Villar, Susana ;
Maria Cortina, Jose .
ANNALS OF THORACIC SURGERY, 2010, 90 (01) :E4-E5
[10]   Revised definitions of invasive fungal disease from the European Organization for Research and Treatment of Cancer/Invasive Fungal Infections Cooperative Group and the National Institute of Allergy and Infectious Diseases Mycoses Study Group (EORTC/MSG) Consensus Group [J].
De Pauw, Ben ;
Walsh, Thomas J. ;
Donnelly, J. Peter ;
Stevens, David A. ;
Edwards, John E. ;
Calandra, Thierry ;
Pappas, Peter G. ;
Maertens, Johan ;
Lortholary, Olivier ;
Kauffman, Carol A. ;
Denning, David W. ;
Patterson, Thomas F. ;
Maschmeyer, Georg ;
Bille, Jacques ;
Dismukes, William E. ;
Herbrecht, Raoul ;
Hope, William W. ;
Kibbler, Christopher C. ;
Kullberg, Bart Jan ;
Marr, Kieren A. ;
Munoz, Patricia ;
Odds, Frank C. ;
Perfect, John R. ;
Restrepo, Angela ;
Ruhnke, Markus ;
Segal, Brahm H. ;
Sobel, Jack D. ;
Sorrell, Tania C. ;
Viscoli, Claudio ;
Wingard, John R. ;
Zaoutis, Theoklis ;
Bennett, John E. .
CLINICAL INFECTIOUS DISEASES, 2008, 46 (12) :1813-1821
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