Multifunctional Regulation of 3D Cell-Laden Microsphere Culture on an Integrated Microfluidic Device

被引：44

作者：

Zheng, Yajing ^{[1
,3
]}

Wu, Zengnan ^{[2
,3
]}

Khan, Mashooq ^{[1
]}

Mao, Sifeng ^{[1
]}

Manibalan, Kesavan ^{[1
]}

Li, Nan ^{[1
]}

Lin, Jin-Ming ^{[1
]}

Lin, Ling ^{[3
]}

机构：

[1] Tsinghua Univ, Dept Chem, MOE Key Lab Bioorgan Phosphorus Chem & Chem Biol, Beijing Key Lab Microanalyt Methods & Instrumenta, Beijing 100084, Peoples R China

[2] Beijing Univ Chem Technol, State Key Lab Chem Resource Engn, Beijing 100029, Peoples R China

[3] Natl Ctr Nanosci & Technol, CAS Ctr Excellence Nanosci, CAS Key Lab Standardizat & Measurement Nanotechno, Beijing 100190, Peoples R China

来源：

ANALYTICAL CHEMISTRY | 2019年 / 91卷 / 19期

基金：

国家重点研发计划; 中国国家自然科学基金;

关键词：

SPHEROID CULTURE; TUMOR; MICROGELS; HYDROGELS; PLATFORM;

D O I：

10.1021/acs.analchem.9b02434

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Three-dimensional (3D) hydrogel micro-spheres have aroused increasing attention as an in vitro cell culture model. Yet the preservation of cells' original biological properties has been overlooked during model construction. Here we present an integrated microfluidic device to accomplish the overall process including cell-laden microsphere generation, online extraction, and dynamic-culture. The method extends the noninvasive and nonsuppression capabilities of the droplet preparation system and provides a constant microenvironment, which reduces intracellular oxidative stress damage and the accumulation of mitochondria. Compared to the conventional preparation method, the coculture model of tumor-endothelial construction on an integrated platform displays high-level angiogenic protein expression. We believe that this versatile and biocompatible platform will provide a more reliable analysis tool for tissue engineering and cancer therapy.

引用

页码：12283 / 12289

页数：7

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