Expression of an endo-(1,3;1,4)-β-glucanase in response to wounding, methyl jasmonate, abscisic acid and ethephon in rice seedlings

被引:32
作者
Akiyama, Takashi [1 ]
Jin, Shigeki [2 ]
Yoshida, Midori [1 ]
Hoshino, Tamotsu [3 ]
Opassiri, Rodjana [4 ]
Cairns, James R. Ketudat [4 ]
机构
[1] Natl Agr Res Ctr Hokkaido Reg, Toyohira Ku, Sapporo, Hokkaido 0628555, Japan
[2] Hokkaido Univ, Grad Sch Hlth Sci, Kita Ku, Sapporo, Hokkaido 0600812, Japan
[3] Natl Res Inst Adv Ind Sci & Technol, Toyohira Ku, Sapporo, Hokkaido 0628517, Japan
[4] Suranaree Univ Technol, Sch Chem & Biochem, Inst Sci, Nakhon Ratchasima 30000, Thailand
关键词
Endo-(1,3,1,4)-beta-glucanase; Leaf elongation; Phytohormone; Rice seedling; Wounding treatment; INDUCED CELL ELONGATION; ORYZA-SATIVA L; GENE-EXPRESSION; HORDEUM-VULGARE; BARLEY; PURIFICATION; SEQUENCE; AUXIN; WALL; ENDOHYDROLASES;
D O I
10.1016/j.jplph.2009.06.002
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
We isolated two rice endo-(1,3;1,4)-beta-glucanase genes, denoted OsEGL1 and OsEGL2, which encoded proteins that shared 64% amino acid sequence identity. Both the OsEGL1 and OsEGL2 genes were successfully expressed in Escherichia coli to produce functional proteins. Purified OsEGL1 and OsEGL2 proteins hydrolyzed (1,3;1,4)-beta-glucans, but not (1,3;1,6)-beta-linked or (1,3)-beta-linked glucopolysaccharides nor carboxymethyl. cellulose, similar to previously characterized grass endo-(1,3;1,4)-beta-glucanases. RNA blot analysis revealed that the OsEGL1 gene is expressed constitutively not only in young roots of rice seedlings, but also in mature roots of adult rice plants. Little or no expression of the OsEGL2 gene was observed in all tissues or treatments tested, but database and RT-PCR analysis indicated it is expressed in ripening panicle. In rice seedling leaves, OsEGL1 gene expression significantly increased in response to methyl jasmonate, abscisic acid, ethephon and mechanical wounding. Mechanical wounding also increased the leaf elongation rate in rice seedlings by 16% relative to that of control seedlings at day 4 after treatment. The increase in the leaf elongation rate of rice seedlings treated under mechanical wounding was concomitant with an increase in OsEGL1 expression levels in seedling leaves. (C) 2009 Elsevier GmbH. All rights reserved.
引用
收藏
页码:1814 / 1825
页数:12
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