Lactate Biosensor Based on Hydrotalcite-Like Compounds: Performances and Application to Serum Samples

被引：13
作者：
Carpani, Irene
Guadagnini, Lorella
Tonelli, Domenica [1 ]
机构：
[1] Univ Bologna, Dept Phys & Inorgan Chem, I-40136 Bologna, Italy
来源：
ELECTROANALYSIS | 2009年 / 21卷 / 22期
关键词：
Lactate; Biosensors; Hydrotalcite; Electrodeposition; Bovine serum; Enzymes; LAYERED DOUBLE HYDROXIDES; GLUCOSE AMPEROMETRIC BIOSENSORS; THIN-FILMS; OXIDASE; DESIGN; ELECTRODES; ELECTROSYNTHESIS; IMMOBILIZATION; DEHYDROGENASE; INTERFERENCE;
D O I：
10.1002/elan.200900118
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A lactate biosensor based on lactate oxidase supported onto a hydrotalcite, electrochemically deposited on a platinum surface, was developed for the first time. For the best electrode configuration, a linear response up to 0.8 mM, with a limit of detection of 14 mu M and a sensitivity of 9 mA M-1 cm(-2), was obtained. The influence of some interferents due to the oxidation of hydrogen peroxide (at +0.35 V vs. SCE) was also studied. By controlling carefully the experimental conditions, the determination of lactate in a commercial serum sample in the presence of interferents was successfully accomplished.
引用
收藏
页码：2401 / 2409
页数：9
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