Genetic diversity analysis of eighty six Indian wheat genotypes was performed using sodium dodecyl sulphate polyacrylamide gel (SDS-PAGE) electrophoresis based on their total seed storage protein. A dendrogram was constructed using the genetic similarity coefficient matrix based on SDS-PAGE, which categorized these genotypes according to their electrophoretic patterns into eight different clusters. These eighty six genotypes studied here belong to three different species of wheat. Out of these eighty six genotypes, two genotypes belong to T. dicoccum, eleven belong to T. durum and the remaining seventy three belongs to T. aestivum. In the dendrogram, all the eleven genotypes of the species T. durum (A-9-30-1, HD-4672, RAJ-1555, PDW-233, PBW-34, HI-8381, PDW-291, WH-896, PDW-215, HI-8498 and MACS-2846) were grouped in one cluster. The pedigree analysis of genotypes showed similar parentage with the electrophoretic profiling of the different wheat genotypes. From this study, it has been concluded that the total seed storage protein profile of wheat can be used as a marker for genetic diversity.