Generation of induced pluripotent stem cells from peripheral blood CD34+hematopoietic progenitors of a 31 year old healthy woman

被引：19

作者：

Tangprasittipap, Amornrat ^{[1
]}

Jittorntrum, Bunyada ^{[1
]}

Wongkummool, Wasinee ^{[2
,3
]}

Kitiyanant, Narisorn ^{[4
]}

Tubsuwan, Alisa ^{[4
]}

机构：

[1] Mahidol Univ, Off Res Acad Affairs & Innovat, Fac Med, Ramathibodi Hosp, Bangkok 10400, Thailand

[2] Mahidol Univ, Inst Mol Biosci, Thalassemia Res Ctr, Nakhon Pathom 73170, Thailand

[3] Mahidol Univ, Fac Med, Dept Biochem, Siriraj Hosp, Bangkok 10700, Thailand

[4] Mahidol Univ, Inst Mol Biosci, Stem Cell Res Grp, Nakhon Pathom 73170, Thailand

来源：

STEM CELL RESEARCH | 2017年 / 20卷

关键词：

D O I：

10.1016/j.scr.2017.02.013

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

The MUi019- A human induced pluripotent stem cell line was generated from peripheral blood CD34+ hematopoietic progenitors of a healthy woman using a non-integrative reprogramming method. Episomal vectors carrying reprogramming factors OCT4, SOX2, KLF4, L-MYC, LIN28, and shRNA of TP53 and EBNA-1 were delivered using electroporation. The iPSC line can be used as a control in studying disease mechanisms. Furthermore, gene editing approaches can be used to introduce specific mutations into the MUi019- A to model disease while the cell type affected by the disease is inaccessible. (C) 2017 The Authors. Published by Elsevier B.V.

引用

页码：91 / 93

页数：3

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