FLC polymerization: Another hurdle towards standardization of FLC measurements

被引：7
作者：
Kaplan, Batia [1 ]
Jacobs, Joannes F. M. [2 ]
机构：
[1] Sheba Med Ctr, Heller Inst Med Res, Ramat Gan, Israel
[2] Radboud Univ Nijmegen, Med Ctr, Dept Lab Med, Lab Med Immunol, Route 469,Geert Grootepl 10, NL-6525 GA Nijmegen, Netherlands
来源：
CLINICA CHIMICA ACTA | 2021年 / 515卷
关键词：
Free light chain; M-protein; Monoclonal gammopathy; Standardization;
D O I：
10.1016/j.cca.2020.12.030
中图分类号：
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号：
1001 ;
摘要：
In the November issue of this journal, Caponi et al. convincingly show that free light chain (FLC) lambda measurements are influenced by the FLC-polymerization status. With liquid chromatography using a size-exclusion column they separated monoclonal FLC dimers from monomers. FLC analysis of these different fractions clearly indicated that Freelite (The Binding Site) reacted better with FLC dimers than with FLC monomers. In contrast, N Latex FLC (Siemens) recognizes mainly FLC monomers and has poor reactivity with the FLC dimers. This important finding may be one of the leading factors to explain discrepancies in FLC-quantification using Freelite versus other immunochemical assays such as N-Latex FLC.
引用
收藏
页码：42 / 43
页数：2
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