Eugenol-Containing Essential Oils Loaded onto Chitosan/Polyvinyl Alcohol Blended Films and Their Ability to Eradicate Staphylococcus aureus or Pseudomonas aeruginosa from Infected Microenvironments

被引:57
作者
Antunes, Joana C. [1 ]
Tavares, Tania D. [1 ]
Teixeira, Marta A. [1 ]
Teixeira, Marta O. [1 ]
Homem, Natalia C. [1 ]
Amorim, M. Teresa P. [1 ]
Felgueiras, Helena P. [1 ]
机构
[1] Univ Minho, Ctr Text Sci & Technol 2C2T, Campus Azurem, P-4800058 Guimaraes, Portugal
关键词
bactericidal; chitosan; essential oils; blended films; wound dressings; wound healing; POLY VINYL ALCOHOL; DIABETIC FOOT INFECTIONS; ANTIMICROBIAL PROPERTIES; POLYVINYL-ALCOHOL; ANTIBACTERIAL PROPERTIES; MECHANICAL-PROPERTIES; LEAVES EXTRACT; CHITOSAN; CINNAMON; CLOVE;
D O I
10.3390/pharmaceutics13020195
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Chronic wounds (CW) create numerous entryways for pathogen invasion and prosperity, further damaging host tissue and hindering its remodeling and repair. Essential oils (EOs) exert quick and efficient antimicrobial (AM) action, unlikely to induce bacterial resistance. Cinnamon leaf and clove oils (CLO and CO) display strong AM activity, namely against Staphylococcus aureus and Pseudomonas aeruginosa. Chitosan (CS) is a natural and biodegradable cationic polysaccharide, also widely known for its AM features. CS and poly (vinyl alcohol) (PVA) films were prepared (ratio 30/70 w/w; 9 wt%) by the solvent casting and phase inversion method. The film's thermal stability and chemical composition data reinforced polymer blending and EO entrapment. Films were supplemented with 1 and 10 wt% of EO in relation to total polymeric mass. The film thickness and degree of swelling (DS) tended to increase with EO content, particularly with 10 wt % CLO (* p < 0.05). UV-visible absorbance scans in the 250-320 cm(-1) region confirmed the successful uptake of CLO and CO into CS/PVA films, particularly with films loaded with 10 wt% EO that contained 5.30/5.32 times more CLO/CO than films supplemented with 1 wt% EO. AM testing revealed that CS films alone were effective against both bacteria and capable of eradicating all P. aeruginosa within the hour (*** p < 0.001). Still, loaded CS/PVA films showed significantly improved AM traits in relation to unloaded films within 2 h of contact. This study is a first proof of concept that CLO and CO can be dispersed into CS/PVA films and show bactericidal effects, particularly against S. aureus, this way paving the way for efficient CW therapeutics.
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页码:1 / 22
页数:22
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共 85 条
[1]   In diabetic foot infections antibiotics are to treat infection, not to heal wounds [J].
Abbas, Mohamed ;
Uckay, Ilker ;
Lipsky, Benjamin A. .
EXPERT OPINION ON PHARMACOTHERAPY, 2015, 16 (06) :821-832
[2]   RETRACTED: Antimicrobial and antioxidant properties of chitosan and its derivatives and their applications: A review (Retracted article. See vol. 293, 2025) [J].
Abd El-Hack, Mohamed E. ;
El-Saadony, Mohamed T. ;
Shafi, Manal E. ;
Zabermawi, Nidal M. ;
Arif, Muhammad ;
Batiha, Gaber Elsaber ;
Khafaga, Asmaa F. ;
Abd El-Hakim, Yasmina M. ;
Al-Sagheer, Adham A. .
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2020, 164 :2726-2744
[3]   Physico-mechanical and antimicrobial properties of gelatin film from the skin of unicorn leatherjacket incorporated with essential oils [J].
Ahmad, Mehraj ;
Benjakul, Soottawat ;
Prodpran, Thummanoon ;
Agustini, Tri Winarni .
FOOD HYDROCOLLOIDS, 2012, 28 (01) :189-199
[4]   Effect of carvacrol in the properties of films based on poly (vinyl alcohol) with different molecular characteristics [J].
Andrade, J. ;
Gonzalez-Martinez, C. ;
Chiralt, Amparo .
POLYMER DEGRADATION AND STABILITY, 2020, 179
[5]  
Antunes J., 2016, Res. Rev. J. Mater. Sci, V4, P12, DOI DOI 10.4172/2321-6212.1000153
[6]   Layer-by-Layer Self-Assembly of Chitosan and Poly(γ-glutamic acid) into Polyelectrolyte Complexes [J].
Antunes, Joana C. ;
Pereira, Catarina Leite ;
Molinos, Maria ;
Ferreira-da-Silva, Frederico ;
Dessi, Mariagemiliana ;
Gloria, Antonio ;
Ambrosio, Luigi ;
Goncalves, Raquel M. ;
Barbosa, Mario A. .
BIOMACROMOLECULES, 2011, 12 (12) :4183-4195
[7]   Evaluation of electrospun poly (vinyl alcohol)-based nanofiber mats incorporated with Zataria multiflora essential oil as potential wound dressing [J].
Ardekani, Niloofar Torabi ;
Khorram, Mohammad ;
Zomorodian, Kamiar ;
Yazdanpanah, Somayeh ;
Veisi, Harried ;
Veisi, Hojat .
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2019, 125 :743-750
[8]   Encapsulation of clove essential oil in hydroxypropyl beta-cyclodextrin for characterization, controlled release, and antioxidant activity [J].
Babaoglu, Humeyra Cetin ;
Bayrak, Ali ;
Ozdemir, Necla ;
Ozgun, Nuriye .
JOURNAL OF FOOD PROCESSING AND PRESERVATION, 2017, 41 (05)
[9]   Clove and eugenol in noncytotoxic concentrations exert immunomodulatory/anti-inflammatory action on cytokine production by murine macrophages [J].
Bachiega, Tatiana Fernanda ;
de Sousa, Joao Paulo Barreto ;
Bastos, Jairo Kenupp ;
Sforcin, Jose Mauricio .
JOURNAL OF PHARMACY AND PHARMACOLOGY, 2012, 64 (04) :610-616
[10]   Biological effects of essential oils - A review [J].
Bakkali, F. ;
Averbeck, S. ;
Averbeck, D. ;
Waomar, M. .
FOOD AND CHEMICAL TOXICOLOGY, 2008, 46 (02) :446-475